Details
| Original language | English |
|---|---|
| Article number | 355 |
| Journal | Journal of Fungi |
| Volume | 8 |
| Issue number | 4 |
| Early online date | 30 Mar 2022 |
| Publication status | Published - Apr 2022 |
Abstract
Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.
Keywords
- heterologous expression, microbial cell factory, PKS, PKS-NRPS, Trichoderma reesei, waste valorization
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Agricultural and Biological Sciences(all)
- Plant Science
- Medicine(all)
- Microbiology (medical)
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In: Journal of Fungi, Vol. 8, No. 4, 355, 04.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization
AU - Shenouda, Mary L.
AU - Ambilika, Maria
AU - Skellam, Elizabeth
AU - Cox, Russell J.
N1 - Funding Information: Funding: M.L.S. thanks the German Academic Exchange Service (DAAD) and the Egyptian Ministry of Higher Education and Scientific Research (MHESR) for funding (GERLS programme, 2017, 57311832). This work was supported by DFG (CO 1328/2-1, INST 187/621-1, INST 187/686-1). The APC was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2022/4
Y1 - 2022/4
N2 - Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.
AB - Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterol-ogous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.
KW - heterologous expression
KW - microbial cell factory
KW - PKS
KW - PKS-NRPS
KW - Trichoderma reesei
KW - waste valorization
UR - http://www.scopus.com/inward/record.url?scp=85128351684&partnerID=8YFLogxK
U2 - 10.3390/jof8040355
DO - 10.3390/jof8040355
M3 - Article
AN - SCOPUS:85128351684
VL - 8
JO - Journal of Fungi
JF - Journal of Fungi
IS - 4
M1 - 355
ER -