Details
| Original language | English |
|---|---|
| Pages (from-to) | 181-189 |
| Number of pages | 9 |
| Journal | Journal of membrane science |
| Volume | 299 |
| Issue number | 1-2 |
| Publication status | Published - 27 Apr 2007 |
Abstract
The broad application of hollow fiber micro-bioreactors in various areas of biotechnology is still restricted due to a limited functionality of the membranes. With this paper a straight forward procedure for membrane functionalization is presented. Different commercially available polyethersulfone (PES) and polysulfone (PS) hollow fiber membranes were chemically modified by reacting terminal hydroxyl groups with ethylene glycol diglycidyl ether (EGDGE) to produce terminal epoxy groups. For increasing loading capacity hydroxyethyl cellulose polymers (HEC) were bound to the epoxy groups. Second epoxidation produced final polymers containing reactive epoxy groups on the hollow fiber surface. From this modified PES and PS, respectively, a wide variety of N-containing reagents (e.g. iminodiacetic acid (IDA)) can be bound to the epoxy groups. The different reactions were proved by acid orange II assay and phenol sulfuric assay. To assure intact hollow fiber surface after membrane modification scanning electron microscopic analyses were performed. The chelating IDA-membranes were complexed with different divalent metal ions (Cu2+, Ni2+, Co2+, Zn2+). Immobilized metal ion affinity PES hollow fiber membranes were used for purification of a recombinant protein (GFP-His) from Escherichia coli, which carried a polyhistidine sequence (His6-tag). Determination of bound and unbound GFP-His6 was performed by fluorescence spectroscopy, Bradford assay and SDS-PAGE.
Keywords
- Epoxidized polyethersulfone/polysulfone, GFP-His, Hollow fiber membranes, Immobilized metal ions, Membrane modification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Materials Science(all)
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemical Engineering(all)
- Filtration and Separation
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In: Journal of membrane science, Vol. 299, No. 1-2, 27.04.2007, p. 181-189.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Heterogeneous surface modification of hollow fiber membranes for use in micro-reactor systems
AU - Kroll, Stephen
AU - Meyer, Lina
AU - Graf, Alain Michel
AU - Beutel, Sascha
AU - Glökler, Jörn
AU - Döring, Stefan
AU - Klaus, Uwe
AU - Scheper, Thomas
N1 - Funding information: Financial support for working on this project was provided by the Deutsche Bundesstiftung Umwelt (DBU), AZ 13120, Osnabrück, Germany.
PY - 2007/4/27
Y1 - 2007/4/27
N2 - The broad application of hollow fiber micro-bioreactors in various areas of biotechnology is still restricted due to a limited functionality of the membranes. With this paper a straight forward procedure for membrane functionalization is presented. Different commercially available polyethersulfone (PES) and polysulfone (PS) hollow fiber membranes were chemically modified by reacting terminal hydroxyl groups with ethylene glycol diglycidyl ether (EGDGE) to produce terminal epoxy groups. For increasing loading capacity hydroxyethyl cellulose polymers (HEC) were bound to the epoxy groups. Second epoxidation produced final polymers containing reactive epoxy groups on the hollow fiber surface. From this modified PES and PS, respectively, a wide variety of N-containing reagents (e.g. iminodiacetic acid (IDA)) can be bound to the epoxy groups. The different reactions were proved by acid orange II assay and phenol sulfuric assay. To assure intact hollow fiber surface after membrane modification scanning electron microscopic analyses were performed. The chelating IDA-membranes were complexed with different divalent metal ions (Cu2+, Ni2+, Co2+, Zn2+). Immobilized metal ion affinity PES hollow fiber membranes were used for purification of a recombinant protein (GFP-His) from Escherichia coli, which carried a polyhistidine sequence (His6-tag). Determination of bound and unbound GFP-His6 was performed by fluorescence spectroscopy, Bradford assay and SDS-PAGE.
AB - The broad application of hollow fiber micro-bioreactors in various areas of biotechnology is still restricted due to a limited functionality of the membranes. With this paper a straight forward procedure for membrane functionalization is presented. Different commercially available polyethersulfone (PES) and polysulfone (PS) hollow fiber membranes were chemically modified by reacting terminal hydroxyl groups with ethylene glycol diglycidyl ether (EGDGE) to produce terminal epoxy groups. For increasing loading capacity hydroxyethyl cellulose polymers (HEC) were bound to the epoxy groups. Second epoxidation produced final polymers containing reactive epoxy groups on the hollow fiber surface. From this modified PES and PS, respectively, a wide variety of N-containing reagents (e.g. iminodiacetic acid (IDA)) can be bound to the epoxy groups. The different reactions were proved by acid orange II assay and phenol sulfuric assay. To assure intact hollow fiber surface after membrane modification scanning electron microscopic analyses were performed. The chelating IDA-membranes were complexed with different divalent metal ions (Cu2+, Ni2+, Co2+, Zn2+). Immobilized metal ion affinity PES hollow fiber membranes were used for purification of a recombinant protein (GFP-His) from Escherichia coli, which carried a polyhistidine sequence (His6-tag). Determination of bound and unbound GFP-His6 was performed by fluorescence spectroscopy, Bradford assay and SDS-PAGE.
KW - Epoxidized polyethersulfone/polysulfone
KW - GFP-His
KW - Hollow fiber membranes
KW - Immobilized metal ions
KW - Membrane modification
UR - http://www.scopus.com/inward/record.url?scp=34250694019&partnerID=8YFLogxK
U2 - 10.1016/j.memsci.2007.04.039
DO - 10.1016/j.memsci.2007.04.039
M3 - Article
AN - SCOPUS:34250694019
VL - 299
SP - 181
EP - 189
JO - Journal of membrane science
JF - Journal of membrane science
SN - 0376-7388
IS - 1-2
ER -