Details
| Original language | English |
|---|---|
| Pages (from-to) | 501-510 |
| Number of pages | 10 |
| Journal | Dental Materials |
| Volume | 35 |
| Issue number | 3 |
| Publication status | Published - Mar 2019 |
| Externally published | Yes |
Abstract
OBJECTIVES: 2-Hydroxyethyl methacrylate (HEMA) is a widely used monomer of dental resin composite materials. Incomplete curing of resins leads to elution of HEMA, which may come in contact with different cells in oral tissues. We aimed to analyze the impact of HEMA on the transcription of genes participating in detoxification of oxidative stress, inflammatory response and organization of the extracellular matrix (ECM) using human gingival fibroblasts (HGFs) and human oral keratinocytes (OKF6/TERT2).
METHODS: Cells were grown in monolayer cultures and treated with different HEMA concentrations (0.5-10mM). H33342 and LDH assays were used to determine HEMA-caused cytotoxicity. Quantitative RT-PCR was used to analyze mRNA expression of four genes related to oxidative stress and five genes each related to inflammation and organization of the ECM.
RESULTS: HEMA caused similar concentration-dependent cytotoxicity in fibroblasts and keratinocytes. Analysis of the transcription showed that genes were regulated in both cell types after HEMA treatment. Genes related to defense against oxidative stress were transcriptionally induced, genes related to inflammation were mainly reduced and genes related to the organization of the ECM were differentially modulated.
SIGNIFICANCE: We analyzed concurrent and HEMA-dependent differential expression of 14 important genes, which have a special significance for cellular processes that are linked to redox and tissue homeostasis. The results suggest that HEMA has an impact on cellular redox-homeostasis with potential impairment of inflammatory responses and of the organization of the ECM in human gingival fibroblasts and oral keratinocytes as first target cells of eluted HEMA.
Keywords
- Extracellular Matrix, Humans, Methacrylates, Oxidation-Reduction, Oxidative Stress, Reactive Oxygen Species, Oxidative stress, Oral keratinocytes, Gingival fibroblasts, Inflammation, Gene expression, ECM, HEMA
ASJC Scopus subject areas
- Engineering(all)
- Mechanics of Materials
- Materials Science(all)
- General Materials Science
- Dentistry(all)
- General Dentistry
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In: Dental Materials, Vol. 35, No. 3, 03.2019, p. 501-510.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - HEMA modulates the transcription of genes related to oxidative defense, inflammatory response and organization of the ECM in human oral cells
AU - Perduns, Renke
AU - Volk, Joachim
AU - Schertl, Peter
AU - Leyhausen, Gabriele
AU - Geurtsen, Werner
N1 - Funding information: Our thanks are due to Ms. S. Wielgosz, Ms. R. Bergin and Ms. A. Beckedorf for their commitment and excellent technical assistance. This study was supported by a grant of the Deutsche Forschungsgemeinschaft/German National Science Foundation (DFG) (VO 1727/1-4). Our thanks are due to Ms. S. Wielgosz, Ms. R. Bergin and Ms. A. Beckedorf for their commitment and excellent technical assistance. This study was supported by a grant of the Deutsche Forschungsgemeinschaft/German National Science Foundation (DFG) (VO 1727/1-4 ).
PY - 2019/3
Y1 - 2019/3
N2 - OBJECTIVES: 2-Hydroxyethyl methacrylate (HEMA) is a widely used monomer of dental resin composite materials. Incomplete curing of resins leads to elution of HEMA, which may come in contact with different cells in oral tissues. We aimed to analyze the impact of HEMA on the transcription of genes participating in detoxification of oxidative stress, inflammatory response and organization of the extracellular matrix (ECM) using human gingival fibroblasts (HGFs) and human oral keratinocytes (OKF6/TERT2).METHODS: Cells were grown in monolayer cultures and treated with different HEMA concentrations (0.5-10mM). H33342 and LDH assays were used to determine HEMA-caused cytotoxicity. Quantitative RT-PCR was used to analyze mRNA expression of four genes related to oxidative stress and five genes each related to inflammation and organization of the ECM.RESULTS: HEMA caused similar concentration-dependent cytotoxicity in fibroblasts and keratinocytes. Analysis of the transcription showed that genes were regulated in both cell types after HEMA treatment. Genes related to defense against oxidative stress were transcriptionally induced, genes related to inflammation were mainly reduced and genes related to the organization of the ECM were differentially modulated.SIGNIFICANCE: We analyzed concurrent and HEMA-dependent differential expression of 14 important genes, which have a special significance for cellular processes that are linked to redox and tissue homeostasis. The results suggest that HEMA has an impact on cellular redox-homeostasis with potential impairment of inflammatory responses and of the organization of the ECM in human gingival fibroblasts and oral keratinocytes as first target cells of eluted HEMA.
AB - OBJECTIVES: 2-Hydroxyethyl methacrylate (HEMA) is a widely used monomer of dental resin composite materials. Incomplete curing of resins leads to elution of HEMA, which may come in contact with different cells in oral tissues. We aimed to analyze the impact of HEMA on the transcription of genes participating in detoxification of oxidative stress, inflammatory response and organization of the extracellular matrix (ECM) using human gingival fibroblasts (HGFs) and human oral keratinocytes (OKF6/TERT2).METHODS: Cells were grown in monolayer cultures and treated with different HEMA concentrations (0.5-10mM). H33342 and LDH assays were used to determine HEMA-caused cytotoxicity. Quantitative RT-PCR was used to analyze mRNA expression of four genes related to oxidative stress and five genes each related to inflammation and organization of the ECM.RESULTS: HEMA caused similar concentration-dependent cytotoxicity in fibroblasts and keratinocytes. Analysis of the transcription showed that genes were regulated in both cell types after HEMA treatment. Genes related to defense against oxidative stress were transcriptionally induced, genes related to inflammation were mainly reduced and genes related to the organization of the ECM were differentially modulated.SIGNIFICANCE: We analyzed concurrent and HEMA-dependent differential expression of 14 important genes, which have a special significance for cellular processes that are linked to redox and tissue homeostasis. The results suggest that HEMA has an impact on cellular redox-homeostasis with potential impairment of inflammatory responses and of the organization of the ECM in human gingival fibroblasts and oral keratinocytes as first target cells of eluted HEMA.
KW - Extracellular Matrix
KW - Humans
KW - Methacrylates
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Reactive Oxygen Species
KW - Oxidative stress
KW - Oral keratinocytes
KW - Gingival fibroblasts
KW - Inflammation
KW - Gene expression
KW - ECM
KW - HEMA
UR - http://www.scopus.com/inward/record.url?scp=85060335721&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2019.01.011
DO - 10.1016/j.dental.2019.01.011
M3 - Article
C2 - 30686707
VL - 35
SP - 501
EP - 510
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 3
ER -