Details
| Original language | English |
|---|---|
| Title of host publication | Recombinant Protein Production in Yeast |
| Editors | Brigitte Gasser, Diethard Mattanovich |
| Place of Publication | New York |
| Pages | 309-322 |
| Number of pages | 14 |
| Edition | 1. |
| ISBN (electronic) | 978-1-4939-9024-5 |
| Publication status | Published - 9 Feb 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1923 |
| ISSN (Print) | 1064-3745 |
| ISSN (electronic) | 1940-6029 |
Abstract
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
Keywords
- HBsAg, Pichia pastoris, Vaccine, Virus, Virus-like particle
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Sustainable Development Goals
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Recombinant Protein Production in Yeast. ed. / Brigitte Gasser; Diethard Mattanovich. 1. ed. New York, 2019. p. 309-322 (Methods in Molecular Biology; Vol. 1923).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Guidelines for small-scale production and purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris
AU - Zahid, Maria
AU - Rinas, Ursula
N1 - Funding information: We are grateful to C. Gurramkonda for critical comments.
PY - 2019/2/9
Y1 - 2019/2/9
N2 - Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
AB - Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
KW - HBsAg
KW - Pichia pastoris
KW - Vaccine
KW - Virus
KW - Virus-like particle
UR - http://www.scopus.com/inward/record.url?scp=85061289955&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9024-5_14
DO - 10.1007/978-1-4939-9024-5_14
M3 - Contribution to book/anthology
C2 - 30737747
AN - SCOPUS:85061289955
SN - 978-1-4939-9023-8
T3 - Methods in Molecular Biology
SP - 309
EP - 322
BT - Recombinant Protein Production in Yeast
A2 - Gasser, Brigitte
A2 - Mattanovich, Diethard
CY - New York
ER -