Details
| Original language | English |
|---|---|
| Pages (from-to) | 17-31 |
| Number of pages | 15 |
| Journal | Stem cell reviews and reports |
| Volume | 7 |
| Issue number | 1 |
| Publication status | Published - Mar 2011 |
Abstract
Up to 2.8 × 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected from separate compartments of UC, display a distinct chondrogenic and adipogenic potential. Therefore they are potential candidates for cartilage and adipose tissue engineering. Cell differentiation along the osteogenic pathway is, however, less efficient, even after the addition of 1.25-dihydroxyvitamin D3, a potent osteoinductive substance. Isolated cells are highly proliferative, tolerate cryopreservation with an average survival rate of about 75% and after thawing can be propagated further, at least over 20 population doublings before their proliferative activity begins to decline. More importantly, they synthesize numerous trophic factors including neurotrophins and factors which facilitate angiogenesis and hematopoiesis. In conclusion, cells isolated from whole UC satisfies all requirements essential for the generation of stem cell banks containing permanently available cell material for applications in the field of regenerative medicine. Nevertheless, further studies are needed to improve and adjust the methods which are already employed for adult MSC expansion and differentiation to specific properties and requirements of the primitive stem cells collected from UC. So, our data verify that the choice of individual parameters for cell propagation, such as duration of cell expansion and cell seeding density, has a substantial impact on the quality of UC-derived cell populations.
Keywords
- Adipogenesis, Adult, Alkaline Phosphatase/metabolism, Cell Count, Cell Differentiation, Cell Proliferation, Cell Survival, Cells, Cultured, Chondrogenesis, Cytokines/metabolism, Flow Cytometry, Humans, Immunophenotyping, Kinetics, Mesoderm/cytology, Osteogenesis, Reproducibility of Results, Stromal Cells/cytology, Time Factors, Umbilical Cord/cytology
Sustainable Development Goals
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In: Stem cell reviews and reports, Vol. 7, No. 1, 03.2011, p. 17-31.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Growth and differentiation properties of mesenchymal stromal cell populations derived from whole human umbilical cord
AU - Majore, Ingrida
AU - Moretti, Pierre
AU - Stahl, Frank
AU - Hass, Ralf
AU - Kasper, Cornelia
N1 - Funding information: This study was supported by a grant from the German Research Foundation (Project number KA 1784/5).
PY - 2011/3
Y1 - 2011/3
N2 - Up to 2.8 × 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected from separate compartments of UC, display a distinct chondrogenic and adipogenic potential. Therefore they are potential candidates for cartilage and adipose tissue engineering. Cell differentiation along the osteogenic pathway is, however, less efficient, even after the addition of 1.25-dihydroxyvitamin D3, a potent osteoinductive substance. Isolated cells are highly proliferative, tolerate cryopreservation with an average survival rate of about 75% and after thawing can be propagated further, at least over 20 population doublings before their proliferative activity begins to decline. More importantly, they synthesize numerous trophic factors including neurotrophins and factors which facilitate angiogenesis and hematopoiesis. In conclusion, cells isolated from whole UC satisfies all requirements essential for the generation of stem cell banks containing permanently available cell material for applications in the field of regenerative medicine. Nevertheless, further studies are needed to improve and adjust the methods which are already employed for adult MSC expansion and differentiation to specific properties and requirements of the primitive stem cells collected from UC. So, our data verify that the choice of individual parameters for cell propagation, such as duration of cell expansion and cell seeding density, has a substantial impact on the quality of UC-derived cell populations.
AB - Up to 2.8 × 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected from separate compartments of UC, display a distinct chondrogenic and adipogenic potential. Therefore they are potential candidates for cartilage and adipose tissue engineering. Cell differentiation along the osteogenic pathway is, however, less efficient, even after the addition of 1.25-dihydroxyvitamin D3, a potent osteoinductive substance. Isolated cells are highly proliferative, tolerate cryopreservation with an average survival rate of about 75% and after thawing can be propagated further, at least over 20 population doublings before their proliferative activity begins to decline. More importantly, they synthesize numerous trophic factors including neurotrophins and factors which facilitate angiogenesis and hematopoiesis. In conclusion, cells isolated from whole UC satisfies all requirements essential for the generation of stem cell banks containing permanently available cell material for applications in the field of regenerative medicine. Nevertheless, further studies are needed to improve and adjust the methods which are already employed for adult MSC expansion and differentiation to specific properties and requirements of the primitive stem cells collected from UC. So, our data verify that the choice of individual parameters for cell propagation, such as duration of cell expansion and cell seeding density, has a substantial impact on the quality of UC-derived cell populations.
KW - Adipogenesis
KW - Adult
KW - Alkaline Phosphatase/metabolism
KW - Cell Count
KW - Cell Differentiation
KW - Cell Proliferation
KW - Cell Survival
KW - Cells, Cultured
KW - Chondrogenesis
KW - Cytokines/metabolism
KW - Flow Cytometry
KW - Humans
KW - Immunophenotyping
KW - Kinetics
KW - Mesoderm/cytology
KW - Osteogenesis
KW - Reproducibility of Results
KW - Stromal Cells/cytology
KW - Time Factors
KW - Umbilical Cord/cytology
U2 - 10.1007/s12015-010-9165-y
DO - 10.1007/s12015-010-9165-y
M3 - Article
C2 - 20596801
VL - 7
SP - 17
EP - 31
JO - Stem cell reviews and reports
JF - Stem cell reviews and reports
SN - 2629-3277
IS - 1
ER -