Details
| Original language | English |
|---|---|
| Pages (from-to) | 104-115 |
| Number of pages | 12 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 148 |
| Publication status | Published - 16 Jun 2016 |
| Externally published | Yes |
Abstract
The use of autologous cells for the coating of implant surfaces presents a promising tool to attenuate foreign body reaction and inflammation. However, insertion forces that occur especially during implantation of electrodes into the narrow cochlea may strip off cells from the surface. Thus, implant surfaces should be ideally structured in a way that protects the cell coating from mechanical removal during implantation. The structuring of implant surfaces may also direct cells towards desired functions to further enhance their performance and clinical suitability. In this study, grid-like square cavities were generated on thermoplastic polyurethane (TPU) surfaces using a combination of femtosecond laser ablation and replication methods. Afterwards, they were tested as potential scaffolds for human bone marrow-derived mesenchymal stem cells (MSCs) in order to use it on neural prostheses. Structured and non-structured TPU allowed proper adhesion and survival of MSCs. Surface structuring resulted in regulation of over 500 genes. Many of the upregulated genes are known to be involved in anti-inflammatory, anti-fibrotic and wound healing processes whereas genes relevant for mesenchymal differentiation programs were downregulated. The enhanced secretion of two representative factors (prostaglandin E2 and interleukin-1 receptor antagonist, respectively) was confirmed by ELISA and the downregulation of other genes involved in adipogenic and osteogenic differentiation were confirmed by gene expression analysis for a cultivation period of up to 21 days. In addition, mRNA of the surface antigens CD24 and ENDOGLIN (CD105) as representative factors for stemness did not show notable variation between cultivation on structured versus non-structured TPU or between 7 versus 21 days of cultivation. Thus, surface topography of TPU seems to be a powerful tool to protect cells from mechanical forces during insertion and to influence cell behaviour.
Keywords
- Inflammation, Mesenchymal stem cell, Neural prosthesis, Polyurethane, Surface topography
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemical Engineering(all)
- Colloid and Surface Chemistry
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Colloids and Surfaces B: Biointerfaces, Vol. 148, 16.06.2016, p. 104-115.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells
AU - Roger, Yvonne
AU - Schäck, Luisa Marilena
AU - Koroleva, Anastasia
AU - Noack, Sandra
AU - Kurselis, Kestutis
AU - Krettek, Christian
AU - Chichkov, Boris
AU - Lenarz, Thomas
AU - Warnecke, Athanasia
AU - Hoffmann, Andrea
N1 - Funding information: We acknowledge the work of Dr. Ramona Weist who investigated CD24 and CD105 expression on and in stem cells during her PhD thesis. These data have been cited as unpublished work in the present manuscript. The skilful technical assistance of Annika Hamm is gratefully acknowledged. The illustration of the graphical abstract was performed by Sabine Gebhardt. Microarray data used or referred to in this publication were generated by the Research Core Unit Transcriptomics of Hannover Medical School. This study was financed by the German Research Foundation, Collaborative Research Centre 599 assigned to B.C., A.H. and A.W., and by DFG HO 2058/4-1, HO 2058/10-1, HO 2058/13-1 to A.H.
PY - 2016/6/16
Y1 - 2016/6/16
N2 - The use of autologous cells for the coating of implant surfaces presents a promising tool to attenuate foreign body reaction and inflammation. However, insertion forces that occur especially during implantation of electrodes into the narrow cochlea may strip off cells from the surface. Thus, implant surfaces should be ideally structured in a way that protects the cell coating from mechanical removal during implantation. The structuring of implant surfaces may also direct cells towards desired functions to further enhance their performance and clinical suitability. In this study, grid-like square cavities were generated on thermoplastic polyurethane (TPU) surfaces using a combination of femtosecond laser ablation and replication methods. Afterwards, they were tested as potential scaffolds for human bone marrow-derived mesenchymal stem cells (MSCs) in order to use it on neural prostheses. Structured and non-structured TPU allowed proper adhesion and survival of MSCs. Surface structuring resulted in regulation of over 500 genes. Many of the upregulated genes are known to be involved in anti-inflammatory, anti-fibrotic and wound healing processes whereas genes relevant for mesenchymal differentiation programs were downregulated. The enhanced secretion of two representative factors (prostaglandin E2 and interleukin-1 receptor antagonist, respectively) was confirmed by ELISA and the downregulation of other genes involved in adipogenic and osteogenic differentiation were confirmed by gene expression analysis for a cultivation period of up to 21 days. In addition, mRNA of the surface antigens CD24 and ENDOGLIN (CD105) as representative factors for stemness did not show notable variation between cultivation on structured versus non-structured TPU or between 7 versus 21 days of cultivation. Thus, surface topography of TPU seems to be a powerful tool to protect cells from mechanical forces during insertion and to influence cell behaviour.
AB - The use of autologous cells for the coating of implant surfaces presents a promising tool to attenuate foreign body reaction and inflammation. However, insertion forces that occur especially during implantation of electrodes into the narrow cochlea may strip off cells from the surface. Thus, implant surfaces should be ideally structured in a way that protects the cell coating from mechanical removal during implantation. The structuring of implant surfaces may also direct cells towards desired functions to further enhance their performance and clinical suitability. In this study, grid-like square cavities were generated on thermoplastic polyurethane (TPU) surfaces using a combination of femtosecond laser ablation and replication methods. Afterwards, they were tested as potential scaffolds for human bone marrow-derived mesenchymal stem cells (MSCs) in order to use it on neural prostheses. Structured and non-structured TPU allowed proper adhesion and survival of MSCs. Surface structuring resulted in regulation of over 500 genes. Many of the upregulated genes are known to be involved in anti-inflammatory, anti-fibrotic and wound healing processes whereas genes relevant for mesenchymal differentiation programs were downregulated. The enhanced secretion of two representative factors (prostaglandin E2 and interleukin-1 receptor antagonist, respectively) was confirmed by ELISA and the downregulation of other genes involved in adipogenic and osteogenic differentiation were confirmed by gene expression analysis for a cultivation period of up to 21 days. In addition, mRNA of the surface antigens CD24 and ENDOGLIN (CD105) as representative factors for stemness did not show notable variation between cultivation on structured versus non-structured TPU or between 7 versus 21 days of cultivation. Thus, surface topography of TPU seems to be a powerful tool to protect cells from mechanical forces during insertion and to influence cell behaviour.
KW - Inflammation
KW - Mesenchymal stem cell
KW - Neural prosthesis
KW - Polyurethane
KW - Surface topography
UR - http://www.scopus.com/inward/record.url?scp=84984827894&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2016.06.024
DO - 10.1016/j.colsurfb.2016.06.024
M3 - Article
C2 - 27591942
AN - SCOPUS:84984827894
VL - 148
SP - 104
EP - 115
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -