Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Abdalrahim Alahmad
  • Wael A. Al-Zereini
  • Tahani J. Hijazin
  • Osama Y. Al-Madanat
  • Ibrahim Alghoraibi
  • Omar Al-Qaralleh
  • Samer Al-Qaraleh
  • Armin Feldhoff
  • Johanna Gabriela Walter
  • Thomas Scheper

External Research Organisations

  • University of Mutah
  • Damascus University
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Details

Original languageEnglish
Article number1104
Number of pages19
JournalPharmaceutics
Volume14
Issue number5
Early online date21 May 2022
Publication statusPublished - May 2022

Abstract

The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in preparing silver nanoparticles (AgNPs) by mixing silver nitrate with hot Hypericum perforatum (St. John’s wort) aqueous extract under high stirring to prevent agglomeration. The formation of silver nanoparticles was monitored by continuous measurement of the surface plasma resonance spectra (UV-VIS). The effect of St. John’s wort aqueous extract on the formation of silver nanoparticles was evaluated and fully characterized by using different physicochemical techniques. The obtained silver nanoparticles were spherical, monodisperse, face-centered cubic (fcc) crystal structures, and the size ranges between 20 to 40 nm. They were covered with a capping layer of organic compounds considered as a nano dimension protective layer that prevents agglomeration and sedimentation. AgNPs revealed antibacterial activity against both tested Gram-positive and Gram-negative bacterial strains causing the formation of 13–32 mm inhibition zones with MIC 6.25–12.5 µg/mL; Escherichia coli strains were resistant to tested AgNPs. The specific growth rate of S. aureus was significantly reduced due to tested AgNPs at concentrations ≥12MIC. AgNPs did not affect wound migration in fibroblast cell lines compared to control. Our results highlighted the potential use of AgNPs capped with plant extracts in the pharmaceutical and food industries to control bacterial pathogens’ growth; however, further studies are required to confirm their wound healing capability and their health impact must be critically evaluated.

Keywords

    antibacterial, green synthesis, Hypericum perforatum L, silver nanoparticles

ASJC Scopus subject areas

Cite this

Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens. / Alahmad, Abdalrahim; Al-Zereini, Wael A.; Hijazin, Tahani J. et al.
In: Pharmaceutics, Vol. 14, No. 5, 1104, 05.2022.

Research output: Contribution to journalArticleResearchpeer review

Alahmad, A., Al-Zereini, W. A., Hijazin, T. J., Al-Madanat, O. Y., Alghoraibi, I., Al-Qaralleh, O., Al-Qaraleh, S., Feldhoff, A., Walter, J. G., & Scheper, T. (2022). Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens. Pharmaceutics, 14(5), Article 1104. https://doi.org/10.3390/pharmaceutics14051104
Alahmad A, Al-Zereini WA, Hijazin TJ, Al-Madanat OY, Alghoraibi I, Al-Qaralleh O et al. Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens. Pharmaceutics. 2022 May;14(5):1104. Epub 2022 May 21. doi: 10.3390/pharmaceutics14051104
Alahmad, Abdalrahim ; Al-Zereini, Wael A. ; Hijazin, Tahani J. et al. / Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens. In: Pharmaceutics. 2022 ; Vol. 14, No. 5.
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title = "Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens",
abstract = "The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in preparing silver nanoparticles (AgNPs) by mixing silver nitrate with hot Hypericum perforatum (St. John{\textquoteright}s wort) aqueous extract under high stirring to prevent agglomeration. The formation of silver nanoparticles was monitored by continuous measurement of the surface plasma resonance spectra (UV-VIS). The effect of St. John{\textquoteright}s wort aqueous extract on the formation of silver nanoparticles was evaluated and fully characterized by using different physicochemical techniques. The obtained silver nanoparticles were spherical, monodisperse, face-centered cubic (fcc) crystal structures, and the size ranges between 20 to 40 nm. They were covered with a capping layer of organic compounds considered as a nano dimension protective layer that prevents agglomeration and sedimentation. AgNPs revealed antibacterial activity against both tested Gram-positive and Gram-negative bacterial strains causing the formation of 13–32 mm inhibition zones with MIC 6.25–12.5 µg/mL; Escherichia coli strains were resistant to tested AgNPs. The specific growth rate of S. aureus was significantly reduced due to tested AgNPs at concentrations ≥12MIC. AgNPs did not affect wound migration in fibroblast cell lines compared to control. Our results highlighted the potential use of AgNPs capped with plant extracts in the pharmaceutical and food industries to control bacterial pathogens{\textquoteright} growth; however, further studies are required to confirm their wound healing capability and their health impact must be critically evaluated.",
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T1 - Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

AU - Alahmad, Abdalrahim

AU - Al-Zereini, Wael A.

AU - Hijazin, Tahani J.

AU - Al-Madanat, Osama Y.

AU - Alghoraibi, Ibrahim

AU - Al-Qaralleh, Omar

AU - Al-Qaraleh, Samer

AU - Feldhoff, Armin

AU - Walter, Johanna Gabriela

AU - Scheper, Thomas

N1 - Funding information: Acknowledgments: The authors are very thankful to Frank Steinbach (Institut für Physikalische Chemie und Elektrochemie, Leibniz Universität Hannover). Financial support from the Avicenna Studienwerk e.V is gratefully acknowledged for providing scholarships for Abdalrahim Alahmad to perform his PhD study. The publication of this article was funded by the Open Access Publishing Fund of Leibniz Universität Hannover.

PY - 2022/5

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N2 - The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in preparing silver nanoparticles (AgNPs) by mixing silver nitrate with hot Hypericum perforatum (St. John’s wort) aqueous extract under high stirring to prevent agglomeration. The formation of silver nanoparticles was monitored by continuous measurement of the surface plasma resonance spectra (UV-VIS). The effect of St. John’s wort aqueous extract on the formation of silver nanoparticles was evaluated and fully characterized by using different physicochemical techniques. The obtained silver nanoparticles were spherical, monodisperse, face-centered cubic (fcc) crystal structures, and the size ranges between 20 to 40 nm. They were covered with a capping layer of organic compounds considered as a nano dimension protective layer that prevents agglomeration and sedimentation. AgNPs revealed antibacterial activity against both tested Gram-positive and Gram-negative bacterial strains causing the formation of 13–32 mm inhibition zones with MIC 6.25–12.5 µg/mL; Escherichia coli strains were resistant to tested AgNPs. The specific growth rate of S. aureus was significantly reduced due to tested AgNPs at concentrations ≥12MIC. AgNPs did not affect wound migration in fibroblast cell lines compared to control. Our results highlighted the potential use of AgNPs capped with plant extracts in the pharmaceutical and food industries to control bacterial pathogens’ growth; however, further studies are required to confirm their wound healing capability and their health impact must be critically evaluated.

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