Details
| Original language | English |
|---|---|
| Title of host publication | Tunable Hydrogels |
| Subtitle of host publication | Smart Materials for Biomedical Applications |
| Place of Publication | Cham |
| Publisher | Springer International Publishing AG |
| Pages | 227-251 |
| Number of pages | 25 |
| ISBN (electronic) | 978-3-030-76769-3 |
| ISBN (print) | 978-3-030-76768-6 |
| Publication status | Published - 2021 |
Publication series
| Name | Advances in Biochemical Engineering/Biotechnology |
|---|---|
| Volume | 178 |
| ISSN (Print) | 0724-6145 |
| ISSN (electronic) | 1616-8542 |
Abstract
Gradient hydrogels represent a pivotal and expanding direction of three-dimensional cell culture. Since various types of gradients play an important role in physiological and pathological processes in vivo, recreation of these gradients in vitro allows a better understanding of cellular behavior, intercellular and cell-matrix interactions. Moreover, gradient hydrogels can advance the creation of functionally improved and physiologically relevant tissue engineered constructs. Another application of gradient hydrogels is the optimization of the 3D in vitro microenvironment (e.g., in terms of hydrogel stiffness or concentration of adhesion ligands). Tunable hydrogels provide researchers with a versatile toolbox to manufacture such gradients in vitro. In this chapter different types of in vivo and in vitro gradients in hydrogels will be presented. Equipment and methods for various gradient fabrications will be discussed. Furthermore, methods of gradient characterizations in hydrogels will be reported. As one of the most recent developments, the influence of low oxygen concentration on cells, as well as the creation and characterization of oxygen gradients in hydrogels will be described. In the last part, achievements in the creation of multiple combinatorial gradients will be presented. The aim of this chapter is to give the reader an overview on existing techniques and biological importance of gradient hydrogels in basic science as well as in applied research.
Keywords
- Gradient hydrogel fabrication, Gradient hydrogels, Hydrogel characterization, In vitro gradient systems, In vivo gradients, Oxygen gradients, Stiffness gradients
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Tunable Hydrogels: Smart Materials for Biomedical Applications. Cham: Springer International Publishing AG, 2021. p. 227-251 (Advances in Biochemical Engineering/Biotechnology; Vol. 178).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research
}
TY - CHAP
T1 - Gradient Hydrogels
AU - Lavrentieva, Antonina
PY - 2021
Y1 - 2021
N2 - Gradient hydrogels represent a pivotal and expanding direction of three-dimensional cell culture. Since various types of gradients play an important role in physiological and pathological processes in vivo, recreation of these gradients in vitro allows a better understanding of cellular behavior, intercellular and cell-matrix interactions. Moreover, gradient hydrogels can advance the creation of functionally improved and physiologically relevant tissue engineered constructs. Another application of gradient hydrogels is the optimization of the 3D in vitro microenvironment (e.g., in terms of hydrogel stiffness or concentration of adhesion ligands). Tunable hydrogels provide researchers with a versatile toolbox to manufacture such gradients in vitro. In this chapter different types of in vivo and in vitro gradients in hydrogels will be presented. Equipment and methods for various gradient fabrications will be discussed. Furthermore, methods of gradient characterizations in hydrogels will be reported. As one of the most recent developments, the influence of low oxygen concentration on cells, as well as the creation and characterization of oxygen gradients in hydrogels will be described. In the last part, achievements in the creation of multiple combinatorial gradients will be presented. The aim of this chapter is to give the reader an overview on existing techniques and biological importance of gradient hydrogels in basic science as well as in applied research.
AB - Gradient hydrogels represent a pivotal and expanding direction of three-dimensional cell culture. Since various types of gradients play an important role in physiological and pathological processes in vivo, recreation of these gradients in vitro allows a better understanding of cellular behavior, intercellular and cell-matrix interactions. Moreover, gradient hydrogels can advance the creation of functionally improved and physiologically relevant tissue engineered constructs. Another application of gradient hydrogels is the optimization of the 3D in vitro microenvironment (e.g., in terms of hydrogel stiffness or concentration of adhesion ligands). Tunable hydrogels provide researchers with a versatile toolbox to manufacture such gradients in vitro. In this chapter different types of in vivo and in vitro gradients in hydrogels will be presented. Equipment and methods for various gradient fabrications will be discussed. Furthermore, methods of gradient characterizations in hydrogels will be reported. As one of the most recent developments, the influence of low oxygen concentration on cells, as well as the creation and characterization of oxygen gradients in hydrogels will be described. In the last part, achievements in the creation of multiple combinatorial gradients will be presented. The aim of this chapter is to give the reader an overview on existing techniques and biological importance of gradient hydrogels in basic science as well as in applied research.
KW - Gradient hydrogel fabrication
KW - Gradient hydrogels
KW - Hydrogel characterization
KW - In vitro gradient systems
KW - In vivo gradients
KW - Oxygen gradients
KW - Stiffness gradients
UR - http://www.scopus.com/inward/record.url?scp=85107349668&partnerID=8YFLogxK
U2 - 10.1007/10_2020_155
DO - 10.1007/10_2020_155
M3 - Contribution to book/anthology
C2 - 33219386
SN - 978-3-030-76768-6
T3 - Advances in Biochemical Engineering/Biotechnology
SP - 227
EP - 251
BT - Tunable Hydrogels
PB - Springer International Publishing AG
CY - Cham
ER -