Details
| Original language | English |
|---|---|
| Article number | 6533 |
| Journal | Scientific Reports |
| Volume | 8 |
| Publication status | Published - 25 Apr 2018 |
Abstract
Stimulation of neuronal cells generally resorts to electric signals. Recent advances in laser-based stimulation methods could present an alternative with superior spatiotemporal resolution. The avoidance of electronic crosstalk makes these methods attractive for in vivo therapeutic application. In particular, nano-mediators, such as gold nanoparticles, can be used to transfer the energy from a laser pulse to the cell membrane and subsequently activate excitable cells. Although the underlying mechanisms of neuronal activation have been widely unraveled, the overall effect on the targeted cell is not understood. Little is known about the physiological and pathophysiological impact of a laser pulse targeted onto nanoabsorbers on the cell membrane. Here, we analyzed the reaction of the neuronal murine cell line Neuro-2A and murine primary cortical neurons to gold nanoparticle mediated laser stimulation. Our study reveals a severe, complex and cell-type independent stress response after laser irradiation, emphasizing the need for a thorough assessment of this approach's efficacy and safety.
Keywords
- Animals, Cell Line, Cell Membrane/drug effects, Gold/pharmacology, Lasers, Metal Nanoparticles/administration & dosage, Mice, Neurons/drug effects
ASJC Scopus subject areas
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In: Scientific Reports, Vol. 8, 6533, 25.04.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Gold nanoparticle-mediated laser stimulation induces a complex stress response in neuronal cells
AU - Johannsmeier, Sonja
AU - Heeger, Patrick
AU - Terakawa, Mitsuhiro
AU - Kalies, Stefan
AU - Heisterkamp, Alexander
AU - Ripken, Tammo
AU - Heinemann, Dag
N1 - Funding information: This work was funded by the DFG Cluster of Excellence EXC 1077/1 “Hearing4all” and supported by the German Academic Exchange Service (DAAD) project-ID 57245147 sponsored by funds of the Federal Ministry of Education and Research (BMBF). S. Kalies and A. Heisterkamp received funding from the German Research Foundation through the Cluster of Excellence REBIRTH (DFG EXC62/3). This work was funded by the DFG Cluster of Excellence EXC 1077/1 Hearing4all and supported by the German Academic Exchange Service (DAAD) project-ID 57245147 sponsored by funds of the Federal Ministry of Education and Research (BMBF). S. Kalies and A. Heisterkamp received funding from the German Research Foundation through the Cluster of Excellence REBIRTH (DFG EXC62/3)
PY - 2018/4/25
Y1 - 2018/4/25
N2 - Stimulation of neuronal cells generally resorts to electric signals. Recent advances in laser-based stimulation methods could present an alternative with superior spatiotemporal resolution. The avoidance of electronic crosstalk makes these methods attractive for in vivo therapeutic application. In particular, nano-mediators, such as gold nanoparticles, can be used to transfer the energy from a laser pulse to the cell membrane and subsequently activate excitable cells. Although the underlying mechanisms of neuronal activation have been widely unraveled, the overall effect on the targeted cell is not understood. Little is known about the physiological and pathophysiological impact of a laser pulse targeted onto nanoabsorbers on the cell membrane. Here, we analyzed the reaction of the neuronal murine cell line Neuro-2A and murine primary cortical neurons to gold nanoparticle mediated laser stimulation. Our study reveals a severe, complex and cell-type independent stress response after laser irradiation, emphasizing the need for a thorough assessment of this approach's efficacy and safety.
AB - Stimulation of neuronal cells generally resorts to electric signals. Recent advances in laser-based stimulation methods could present an alternative with superior spatiotemporal resolution. The avoidance of electronic crosstalk makes these methods attractive for in vivo therapeutic application. In particular, nano-mediators, such as gold nanoparticles, can be used to transfer the energy from a laser pulse to the cell membrane and subsequently activate excitable cells. Although the underlying mechanisms of neuronal activation have been widely unraveled, the overall effect on the targeted cell is not understood. Little is known about the physiological and pathophysiological impact of a laser pulse targeted onto nanoabsorbers on the cell membrane. Here, we analyzed the reaction of the neuronal murine cell line Neuro-2A and murine primary cortical neurons to gold nanoparticle mediated laser stimulation. Our study reveals a severe, complex and cell-type independent stress response after laser irradiation, emphasizing the need for a thorough assessment of this approach's efficacy and safety.
KW - Animals
KW - Cell Line
KW - Cell Membrane/drug effects
KW - Gold/pharmacology
KW - Lasers
KW - Metal Nanoparticles/administration & dosage
KW - Mice
KW - Neurons/drug effects
UR - http://www.scopus.com/inward/record.url?scp=85046000223&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-24908-9
DO - 10.1038/s41598-018-24908-9
M3 - Article
C2 - 29695746
AN - SCOPUS:85046000223
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 6533
ER -