Details
| Original language | English |
|---|---|
| Title of host publication | Conference Proceedings - European Conference on Biomedical Optics, ECBO 2013 |
| Publisher | OSA - The Optical Society |
| ISBN (print) | 9780819496461 |
| Publication status | Published - 12 May 2013 |
| Externally published | Yes |
| Event | European Conference on Biomedical Optics, ECBO 2013 - Munich, Germany Duration: 12 May 2013 → 16 May 2013 |
Publication series
| Name | Optics InfoBase Conference Papers |
|---|---|
| ISSN (electronic) | 2162-2701 |
Abstract
The delivery of antisense structures, like siRNA, is beneficial for new therapeutic approaches in regenerative sciences. Optical transfection techniques enable high spatial control combined with minimal invasive treatment of cells due to the use of short laser pulses. However, single cell laser transfection by a tightly focused laser beam, for example femtosecond laser transfection, has the major drawback of low throughput. Compared to this, high-throughput in laser transfection is possible by applying gold nanoparticles irradiated by a weakly focused laser beam scanning over the cell sample. Herein, we show the delivery of antisense molecules and demonstrate the minimal cytotoxicity of a method called gold nanoparticle mediated (GNOME) laser transfection. A 532 nm microchip laser in conjugation with 200 nm gold nanoparticles at a concentration of 0.5 μg/cmï 2 is used. In addition to antisense molecules, the uptake of dextrans of several sizes is analyzed.
Keywords
- Gene silencing/knockdown, Gold nanoparticle, High throughput, Laser transfection, Minimal invasive
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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Conference Proceedings - European Conference on Biomedical Optics, ECBO 2013. OSA - The Optical Society, 2013. (Optics InfoBase Conference Papers).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Gold nanoparticle mediated laser transfection for high-throughput antisense applications
AU - Kalies, S.
AU - Heinemann, D.
AU - Schomaker, M.
AU - Birr, T.
AU - Ripken, T.
AU - Meyer, H.
PY - 2013/5/12
Y1 - 2013/5/12
N2 - The delivery of antisense structures, like siRNA, is beneficial for new therapeutic approaches in regenerative sciences. Optical transfection techniques enable high spatial control combined with minimal invasive treatment of cells due to the use of short laser pulses. However, single cell laser transfection by a tightly focused laser beam, for example femtosecond laser transfection, has the major drawback of low throughput. Compared to this, high-throughput in laser transfection is possible by applying gold nanoparticles irradiated by a weakly focused laser beam scanning over the cell sample. Herein, we show the delivery of antisense molecules and demonstrate the minimal cytotoxicity of a method called gold nanoparticle mediated (GNOME) laser transfection. A 532 nm microchip laser in conjugation with 200 nm gold nanoparticles at a concentration of 0.5 μg/cmï 2 is used. In addition to antisense molecules, the uptake of dextrans of several sizes is analyzed.
AB - The delivery of antisense structures, like siRNA, is beneficial for new therapeutic approaches in regenerative sciences. Optical transfection techniques enable high spatial control combined with minimal invasive treatment of cells due to the use of short laser pulses. However, single cell laser transfection by a tightly focused laser beam, for example femtosecond laser transfection, has the major drawback of low throughput. Compared to this, high-throughput in laser transfection is possible by applying gold nanoparticles irradiated by a weakly focused laser beam scanning over the cell sample. Herein, we show the delivery of antisense molecules and demonstrate the minimal cytotoxicity of a method called gold nanoparticle mediated (GNOME) laser transfection. A 532 nm microchip laser in conjugation with 200 nm gold nanoparticles at a concentration of 0.5 μg/cmï 2 is used. In addition to antisense molecules, the uptake of dextrans of several sizes is analyzed.
KW - Gene silencing/knockdown
KW - Gold nanoparticle
KW - High throughput
KW - Laser transfection
KW - Minimal invasive
UR - http://www.scopus.com/inward/record.url?scp=84899764073&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84899764073
SN - 9780819496461
T3 - Optics InfoBase Conference Papers
BT - Conference Proceedings - European Conference on Biomedical Optics, ECBO 2013
PB - OSA - The Optical Society
T2 - European Conference on Biomedical Optics, ECBO 2013
Y2 - 12 May 2013 through 16 May 2013
ER -