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Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions

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Authors

  • Anke Becker
  • Hélène Bergès
  • Elizaveta Krol
  • Claude Bruand
  • Helge Küster

External Research Organisations

  • Bielefeld University
  • Institut national de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE)
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    • Citation Indexes: 217
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    • References: 1
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Details

Original languageEnglish
Pages (from-to)292-303
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume17
Issue number3
Publication statusPublished - Mar 2004
Externally publishedYes

Abstract

Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.

Keywords

    Macroarray, Root nodule

ASJC Scopus subject areas

Cite this

Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. / Becker, Anke; Bergès, Hélène; Krol, Elizaveta et al.
In: Molecular Plant-Microbe Interactions, Vol. 17, No. 3, 03.2004, p. 292-303.

Research output: Contribution to journalArticleResearchpeer review

Becker, A, Bergès, H, Krol, E, Bruand, C, Rüberg, S, Capela, D, Lauber, E, Meilhoc, E, Ampe, F, De Bruijn, FJ, Fourment, J, Francez-Charlot, A, Kahn, D, Küster, H, Liebe, C, Pühler, A, Weidner, S & Batut, J 2004, 'Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions', Molecular Plant-Microbe Interactions, vol. 17, no. 3, pp. 292-303. https://doi.org/10.1094/MPMI.2004.17.3.292
Becker, A., Bergès, H., Krol, E., Bruand, C., Rüberg, S., Capela, D., Lauber, E., Meilhoc, E., Ampe, F., De Bruijn, F. J., Fourment, J., Francez-Charlot, A., Kahn, D., Küster, H., Liebe, C., Pühler, A., Weidner, S., & Batut, J. (2004). Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Molecular Plant-Microbe Interactions, 17(3), 292-303. https://doi.org/10.1094/MPMI.2004.17.3.292
Becker A, Bergès H, Krol E, Bruand C, Rüberg S, Capela D et al. Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Molecular Plant-Microbe Interactions. 2004 Mar;17(3):292-303. doi: 10.1094/MPMI.2004.17.3.292
Becker, Anke ; Bergès, Hélène ; Krol, Elizaveta et al. / Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. In: Molecular Plant-Microbe Interactions. 2004 ; Vol. 17, No. 3. pp. 292-303.
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abstract = "Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.",
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AU - Becker, Anke

AU - Bergès, Hélène

AU - Krol, Elizaveta

AU - Bruand, Claude

AU - Rüberg, Silvia

AU - Capela, Delphine

AU - Lauber, Emmanuelle

AU - Meilhoc, Eliane

AU - Ampe, Frédéric

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AU - Fourment, Joëlle

AU - Francez-Charlot, Anne

AU - Kahn, Daniel

AU - Küster, Helge

AU - Liebe, Carine

AU - Pühler, Alfred

AU - Weidner, Stefan

AU - Batut, Jacques

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