Details
Original language | English |
---|---|
Pages (from-to) | 292-303 |
Number of pages | 12 |
Journal | Molecular Plant-Microbe Interactions |
Volume | 17 |
Issue number | 3 |
Publication status | Published - Mar 2004 |
Externally published | Yes |
Abstract
Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.
Keywords
- Macroarray, Root nodule
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
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In: Molecular Plant-Microbe Interactions, Vol. 17, No. 3, 03.2004, p. 292-303.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions
AU - Becker, Anke
AU - Bergès, Hélène
AU - Krol, Elizaveta
AU - Bruand, Claude
AU - Rüberg, Silvia
AU - Capela, Delphine
AU - Lauber, Emmanuelle
AU - Meilhoc, Eliane
AU - Ampe, Frédéric
AU - De Bruijn, Frans J.
AU - Fourment, Joëlle
AU - Francez-Charlot, Anne
AU - Kahn, Daniel
AU - Küster, Helge
AU - Liebe, Carine
AU - Pühler, Alfred
AU - Weidner, Stefan
AU - Batut, Jacques
PY - 2004/3
Y1 - 2004/3
N2 - Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.
AB - Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.
KW - Macroarray
KW - Root nodule
UR - http://www.scopus.com/inward/record.url?scp=12144290956&partnerID=8YFLogxK
U2 - 10.1094/MPMI.2004.17.3.292
DO - 10.1094/MPMI.2004.17.3.292
M3 - Article
C2 - 15000396
AN - SCOPUS:12144290956
VL - 17
SP - 292
EP - 303
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
SN - 0894-0282
IS - 3
ER -