Details
| Original language | English |
|---|---|
| Pages (from-to) | 1093-1097 |
| Number of pages | 5 |
| Journal | HORTSCIENCE |
| Volume | 39 |
| Issue number | 5 |
| Publication status | Published - Aug 2004 |
Abstract
Somatic embryos of cyclamen [Cyclamen persicum Mill.] were produced using a liquid culture system. Two encapsulation techniques, conventional alginate beads and alginate hollow beads, were tested for globular cyclamen somatic embryos with the aim of developing synthetic seeds. Final germination from alginate beads was as high as observed for non encapsulated control embryos (97%), but germination was delayed. In contrast, germination from hollow beads was lower (71%) and occurred later. In hollow beads somatic embryos developed within the capsule, and outgrowth seemed to be more difficult than from alginate. Storage at 4°C for four weeks resulted in a reduction of viability for controls as well as for encapsulated embryos. Incorporation of medium into the capsules improved the speed of germination for both capsule types. However, somatic embryos were not able to germinate on a medium-free support, even if encapsulated in beads containing medium.
Keywords
- Alginate, Artificial seeds, Hollow beads, Storage, Synthetic seeds
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: HORTSCIENCE, Vol. 39, No. 5, 08.2004, p. 1093-1097.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Germination of encapsulated somatic embryos of Cyclamen persicum
AU - Winkelmann, Traud
AU - Meyer, Lara
AU - Serek, Margrethe
PY - 2004/8
Y1 - 2004/8
N2 - Somatic embryos of cyclamen [Cyclamen persicum Mill.] were produced using a liquid culture system. Two encapsulation techniques, conventional alginate beads and alginate hollow beads, were tested for globular cyclamen somatic embryos with the aim of developing synthetic seeds. Final germination from alginate beads was as high as observed for non encapsulated control embryos (97%), but germination was delayed. In contrast, germination from hollow beads was lower (71%) and occurred later. In hollow beads somatic embryos developed within the capsule, and outgrowth seemed to be more difficult than from alginate. Storage at 4°C for four weeks resulted in a reduction of viability for controls as well as for encapsulated embryos. Incorporation of medium into the capsules improved the speed of germination for both capsule types. However, somatic embryos were not able to germinate on a medium-free support, even if encapsulated in beads containing medium.
AB - Somatic embryos of cyclamen [Cyclamen persicum Mill.] were produced using a liquid culture system. Two encapsulation techniques, conventional alginate beads and alginate hollow beads, were tested for globular cyclamen somatic embryos with the aim of developing synthetic seeds. Final germination from alginate beads was as high as observed for non encapsulated control embryos (97%), but germination was delayed. In contrast, germination from hollow beads was lower (71%) and occurred later. In hollow beads somatic embryos developed within the capsule, and outgrowth seemed to be more difficult than from alginate. Storage at 4°C for four weeks resulted in a reduction of viability for controls as well as for encapsulated embryos. Incorporation of medium into the capsules improved the speed of germination for both capsule types. However, somatic embryos were not able to germinate on a medium-free support, even if encapsulated in beads containing medium.
KW - Alginate
KW - Artificial seeds
KW - Hollow beads
KW - Storage
KW - Synthetic seeds
UR - http://www.scopus.com/inward/record.url?scp=3142708065&partnerID=8YFLogxK
U2 - 10.21273/hortsci.39.5.1093
DO - 10.21273/hortsci.39.5.1093
M3 - Article
AN - SCOPUS:3142708065
VL - 39
SP - 1093
EP - 1097
JO - HORTSCIENCE
JF - HORTSCIENCE
SN - 0018-5345
IS - 5
ER -