Details
| Original language | English |
|---|---|
| Pages (from-to) | 75-86 |
| Number of pages | 12 |
| Journal | Phyton - Annales Rei Botanicae |
| Volume | 41 |
| Issue number | 1 |
| Publication status | Published - 1 Jan 2001 |
Abstract
The sulfur supply of agricultural crops plays an important role in their defence strategies against pathogens. The mechanisms of sulfur induced resistance (SIR) are, however, not yet known. The working hypothesis was the involvement of volatile sulfur compounds in SIR, especially the evolution of H2S. In total, ten Brassica lines (no. 1-10) of winter oilseed rape showing a broad variation with view to their genetic pedigree were selected from a set of about 100 genotypes, and leaf samples were taken just before flowering. The activities of a H2S releasing L-cysteine desulfhydrase-like enzyme (EC 4.4.1.-) and of O-acetylserine(thiol)lyase (OAS-TL, EC 4.2.99.8) were measured, and the total sulfur content was determined by X-ray spectroscopy. The higher the sulfur content in plants, the lower was the L-cysteine desulfhydrase activity and the higher was the OAS-TL activity. This is the first time that a correlation was found between enzymatic H2S evolution from L-cysteine and the total sulfur content which is an indicator for the sulfur nutritional status of the crops. The lines showing the strongest significant differences were selected for further investigations.
Keywords
- Brassica napus, Cysteine, Desulfhydrase, HS, O-acetylserine(thiol)lyase, Sulfur induced resistance
ASJC Scopus subject areas
- Environmental Science(all)
- Global and Planetary Change
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Phyton - Annales Rei Botanicae, Vol. 41, No. 1, 01.01.2001, p. 75-86.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genotypical differences in total sulfur contents and cysteine desulfhydrase activities in Brassica napus L.
AU - Burandt, P.
AU - Papenbrock, J.
AU - Schmidt, A.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - The sulfur supply of agricultural crops plays an important role in their defence strategies against pathogens. The mechanisms of sulfur induced resistance (SIR) are, however, not yet known. The working hypothesis was the involvement of volatile sulfur compounds in SIR, especially the evolution of H2S. In total, ten Brassica lines (no. 1-10) of winter oilseed rape showing a broad variation with view to their genetic pedigree were selected from a set of about 100 genotypes, and leaf samples were taken just before flowering. The activities of a H2S releasing L-cysteine desulfhydrase-like enzyme (EC 4.4.1.-) and of O-acetylserine(thiol)lyase (OAS-TL, EC 4.2.99.8) were measured, and the total sulfur content was determined by X-ray spectroscopy. The higher the sulfur content in plants, the lower was the L-cysteine desulfhydrase activity and the higher was the OAS-TL activity. This is the first time that a correlation was found between enzymatic H2S evolution from L-cysteine and the total sulfur content which is an indicator for the sulfur nutritional status of the crops. The lines showing the strongest significant differences were selected for further investigations.
AB - The sulfur supply of agricultural crops plays an important role in their defence strategies against pathogens. The mechanisms of sulfur induced resistance (SIR) are, however, not yet known. The working hypothesis was the involvement of volatile sulfur compounds in SIR, especially the evolution of H2S. In total, ten Brassica lines (no. 1-10) of winter oilseed rape showing a broad variation with view to their genetic pedigree were selected from a set of about 100 genotypes, and leaf samples were taken just before flowering. The activities of a H2S releasing L-cysteine desulfhydrase-like enzyme (EC 4.4.1.-) and of O-acetylserine(thiol)lyase (OAS-TL, EC 4.2.99.8) were measured, and the total sulfur content was determined by X-ray spectroscopy. The higher the sulfur content in plants, the lower was the L-cysteine desulfhydrase activity and the higher was the OAS-TL activity. This is the first time that a correlation was found between enzymatic H2S evolution from L-cysteine and the total sulfur content which is an indicator for the sulfur nutritional status of the crops. The lines showing the strongest significant differences were selected for further investigations.
KW - Brassica napus
KW - Cysteine
KW - Desulfhydrase
KW - HS
KW - O-acetylserine(thiol)lyase
KW - Sulfur induced resistance
UR - http://www.scopus.com/inward/record.url?scp=0034958278&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0034958278
VL - 41
SP - 75
EP - 86
JO - Phyton - Annales Rei Botanicae
JF - Phyton - Annales Rei Botanicae
SN - 0079-2047
IS - 1
ER -