Details
| Original language | English |
|---|---|
| Pages (from-to) | 27-32 |
| Number of pages | 6 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 89 |
| Issue number | 1-2 |
| Publication status | Published - 21 Jan 2003 |
Abstract
A 2D-spectroscopic genotoxicity biosensing system is presented. This optical sensing system monitors the genotoxicant-induced expression of green fluorescent protein (GFP) in a recombinant Escherichia coli strain. In this organism, GFP synthesis is driven by the recA gene promoter of the bacterial SOS DNA repair system. Using nalidixic acid as a model genotoxicant, we have demonstrated a response range that varies as a function of the excitation wavelength. When GFP fluorescence was monitored at 510 nm, the linear working range to nalidixic acid was within the range of 0.03-0.3 ppm at 390 nm excitation and 0.05-0.3 ppm at 480 nm excitation.
Keywords
- 2D-spectroscopy, Genotoxicity, Green fluorescent protein, Whole cell biosensor
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
- Materials Science(all)
- Materials Chemistry
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In: Sensors and Actuators, B: Chemical, Vol. 89, No. 1-2, 21.01.2003, p. 27-32.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genotoxicity monitoring using a 2D-spectroscopic GFP whole cell biosensing system
AU - Bartolome, Amelita J.
AU - Ulber, Roland
AU - Scheper, Thomas
AU - Sagi, Eran
AU - Belkin, Shimshon
N1 - Funding information: A.J.B. wishes to thank the Deustche Akademischer Austauschdienst (DAAD) for the PhD scholarship grant. This project was supported by a research grant from the Land Niedersachen Min. f. Wissenchaft & Kultur 16.11.1998—25 A.5—76 251-99-2/98 (ZN549) to T.S. and S.B.
PY - 2003/1/21
Y1 - 2003/1/21
N2 - A 2D-spectroscopic genotoxicity biosensing system is presented. This optical sensing system monitors the genotoxicant-induced expression of green fluorescent protein (GFP) in a recombinant Escherichia coli strain. In this organism, GFP synthesis is driven by the recA gene promoter of the bacterial SOS DNA repair system. Using nalidixic acid as a model genotoxicant, we have demonstrated a response range that varies as a function of the excitation wavelength. When GFP fluorescence was monitored at 510 nm, the linear working range to nalidixic acid was within the range of 0.03-0.3 ppm at 390 nm excitation and 0.05-0.3 ppm at 480 nm excitation.
AB - A 2D-spectroscopic genotoxicity biosensing system is presented. This optical sensing system monitors the genotoxicant-induced expression of green fluorescent protein (GFP) in a recombinant Escherichia coli strain. In this organism, GFP synthesis is driven by the recA gene promoter of the bacterial SOS DNA repair system. Using nalidixic acid as a model genotoxicant, we have demonstrated a response range that varies as a function of the excitation wavelength. When GFP fluorescence was monitored at 510 nm, the linear working range to nalidixic acid was within the range of 0.03-0.3 ppm at 390 nm excitation and 0.05-0.3 ppm at 480 nm excitation.
KW - 2D-spectroscopy
KW - Genotoxicity
KW - Green fluorescent protein
KW - Whole cell biosensor
UR - http://www.scopus.com/inward/record.url?scp=0037366371&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(02)00423-9
DO - 10.1016/S0925-4005(02)00423-9
M3 - Article
AN - SCOPUS:0037366371
VL - 89
SP - 27
EP - 32
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1-2
ER -