Details
| Original language | English |
|---|---|
| Pages (from-to) | 200-205 |
| Number of pages | 6 |
| Journal | Current opinion in biotechnology |
| Volume | 14 |
| Issue number | 2 |
| Publication status | Published - Apr 2003 |
| Externally published | Yes |
Abstract
Following an interaction with rhizobial soil bacteria, legume plants are able to form a novel organ, termed the root nodule. This organ houses the rhizobial microsymbionts, which perform the biological nitrogen fixation process resulting in the incorporation of ammonia into plant organic molecules. Recent advances in genomics have opened exciting new perspectives in this field by providing the complete gene inventory of two rhizobial microsymbionts. The complete genome sequences of Mesorhizobium loti, the symbiont of several Lotus species, and Sinorhizobium meliloti, the symbiont of alfalfa, were determined and annotated in detail. For legume macrosymbionts, expressed sequence tag projects and expression analyses using DNA arrays in conjunction with proteomics approaches have identified numerous genes involved in root nodule formation and nitrogen fixation. The isolation of legume genes by tagging or positional cloning recently allowed the identification of genes that control the very early steps of root nodule organogenesis.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: Current opinion in biotechnology, Vol. 14, No. 2, 04.2003, p. 200-205.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Genomics insights into symbiotic nitrogen fixation
AU - Weidner, Stefan
AU - Pühler, Alfred
AU - Küster, Helge
N1 - Funding information: The authors acknowledge financial support from the European Union projects MEDICAGO (QLG2-CT-2000-00676; http://medicago.toulouse.inra.fr/EU ) and MELILO (BIO4-CT98-0109), from the Deutsche Forschungsgemeinschaft project MolMyk (Ku1478/1-2; http://www.molmyk.genetik.uni-bielefeld.de ) and from the Bundesministerium für Forschung und Technologie (0311752).
PY - 2003/4
Y1 - 2003/4
N2 - Following an interaction with rhizobial soil bacteria, legume plants are able to form a novel organ, termed the root nodule. This organ houses the rhizobial microsymbionts, which perform the biological nitrogen fixation process resulting in the incorporation of ammonia into plant organic molecules. Recent advances in genomics have opened exciting new perspectives in this field by providing the complete gene inventory of two rhizobial microsymbionts. The complete genome sequences of Mesorhizobium loti, the symbiont of several Lotus species, and Sinorhizobium meliloti, the symbiont of alfalfa, were determined and annotated in detail. For legume macrosymbionts, expressed sequence tag projects and expression analyses using DNA arrays in conjunction with proteomics approaches have identified numerous genes involved in root nodule formation and nitrogen fixation. The isolation of legume genes by tagging or positional cloning recently allowed the identification of genes that control the very early steps of root nodule organogenesis.
AB - Following an interaction with rhizobial soil bacteria, legume plants are able to form a novel organ, termed the root nodule. This organ houses the rhizobial microsymbionts, which perform the biological nitrogen fixation process resulting in the incorporation of ammonia into plant organic molecules. Recent advances in genomics have opened exciting new perspectives in this field by providing the complete gene inventory of two rhizobial microsymbionts. The complete genome sequences of Mesorhizobium loti, the symbiont of several Lotus species, and Sinorhizobium meliloti, the symbiont of alfalfa, were determined and annotated in detail. For legume macrosymbionts, expressed sequence tag projects and expression analyses using DNA arrays in conjunction with proteomics approaches have identified numerous genes involved in root nodule formation and nitrogen fixation. The isolation of legume genes by tagging or positional cloning recently allowed the identification of genes that control the very early steps of root nodule organogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0038330681&partnerID=8YFLogxK
U2 - 10.1016/S0958-1669(03)00022-3
DO - 10.1016/S0958-1669(03)00022-3
M3 - Review article
C2 - 12732321
AN - SCOPUS:0038330681
VL - 14
SP - 200
EP - 205
JO - Current opinion in biotechnology
JF - Current opinion in biotechnology
SN - 0958-1669
IS - 2
ER -