Details
| Original language | English |
|---|---|
| Article number | 25072 |
| Journal | Scientific reports |
| Volume | 14 |
| Issue number | 1 |
| Publication status | Published - 23 Oct 2024 |
Abstract
Adventitious root (AR) formation is a genetically complex trait with high genotypic variability. Therefore, only a limited range of cultivars are currently propagated by cuttings in rose. In this study, we analysed the anatomy of in vitro shoots, the early formation of root primordia (RP) and the formation of ARs in a diverse set of 106 rose genotypes. Correlation analysis indicated that the growth in shoot diameter and the vasculature dimensions after 1 week of rooting contributed to successful AR formation. Using phenotypic data for genome-wide association studies (GWAS) analyses, nine significantly associated single nucleotide polymorphisms (SNPs) and genomic regions contributing to various RP and AR formation traits were identified. The contribution of genomic regions to trait variation was notably greater for traits associated with earlier processes than for traits associated with later developmental stages. The combination of RP and AR data allowed the detection of regions by GWAS that contain factors that potentially limit RP emergence. Homologues of 47 genes known to be involved in AR formation from the literature could be assigned to the identified peaks. Further studies are needed to investigate the suitability of SNPs exhibiting strong effects as allele-specific PCR markers for use in breeding.
Keywords
- Association mapping, Genome-wide association study, Histology, Phloem, Rooting, Rosa × hybrida, Xylem
ASJC Scopus subject areas
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In: Scientific reports, Vol. 14, No. 1, 25072, 23.10.2024.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genome-wide association study (GWAS) analyses of early anatomical changes in rose adventitious root formation
AU - Wamhoff, David
AU - Marxen, Annina
AU - Acharya, Bhawana
AU - Grzelak, Monika
AU - Debener, Thomas
AU - Winkelmann, Traud
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024/10/23
Y1 - 2024/10/23
N2 - Adventitious root (AR) formation is a genetically complex trait with high genotypic variability. Therefore, only a limited range of cultivars are currently propagated by cuttings in rose. In this study, we analysed the anatomy of in vitro shoots, the early formation of root primordia (RP) and the formation of ARs in a diverse set of 106 rose genotypes. Correlation analysis indicated that the growth in shoot diameter and the vasculature dimensions after 1 week of rooting contributed to successful AR formation. Using phenotypic data for genome-wide association studies (GWAS) analyses, nine significantly associated single nucleotide polymorphisms (SNPs) and genomic regions contributing to various RP and AR formation traits were identified. The contribution of genomic regions to trait variation was notably greater for traits associated with earlier processes than for traits associated with later developmental stages. The combination of RP and AR data allowed the detection of regions by GWAS that contain factors that potentially limit RP emergence. Homologues of 47 genes known to be involved in AR formation from the literature could be assigned to the identified peaks. Further studies are needed to investigate the suitability of SNPs exhibiting strong effects as allele-specific PCR markers for use in breeding.
AB - Adventitious root (AR) formation is a genetically complex trait with high genotypic variability. Therefore, only a limited range of cultivars are currently propagated by cuttings in rose. In this study, we analysed the anatomy of in vitro shoots, the early formation of root primordia (RP) and the formation of ARs in a diverse set of 106 rose genotypes. Correlation analysis indicated that the growth in shoot diameter and the vasculature dimensions after 1 week of rooting contributed to successful AR formation. Using phenotypic data for genome-wide association studies (GWAS) analyses, nine significantly associated single nucleotide polymorphisms (SNPs) and genomic regions contributing to various RP and AR formation traits were identified. The contribution of genomic regions to trait variation was notably greater for traits associated with earlier processes than for traits associated with later developmental stages. The combination of RP and AR data allowed the detection of regions by GWAS that contain factors that potentially limit RP emergence. Homologues of 47 genes known to be involved in AR formation from the literature could be assigned to the identified peaks. Further studies are needed to investigate the suitability of SNPs exhibiting strong effects as allele-specific PCR markers for use in breeding.
KW - Association mapping
KW - Genome-wide association study
KW - Histology
KW - Phloem
KW - Rooting
KW - Rosa × hybrida
KW - Xylem
UR - http://www.scopus.com/inward/record.url?scp=85207407524&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-75502-1
DO - 10.1038/s41598-024-75502-1
M3 - Article
C2 - 39443540
AN - SCOPUS:85207407524
VL - 14
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 25072
ER -