Details
| Original language | English |
|---|---|
| Pages (from-to) | 5170-5174 |
| Number of pages | 5 |
| Journal | Applied and Environmental Microbiology |
| Volume | 75 |
| Issue number | 15 |
| Publication status | Published - Aug 2009 |
| Externally published | Yes |
Abstract
Ninety percent of cultured bacterial nitrate reducers with a 16S rRNA gene similarity of ≥97% had a narG or nosZ similarity of ≥67% or ≥80%, respectively, suggesting that 67% and 80% could be used as standardized, conservative threshold similarity values for narG and nosZ, respectively (i.e., any two sequences that are less similar than the threshold similarity value have a very high probability of belonging to different species), for estimating species-level operational taxonomic units. Genus-level tree topologies of narG and nosZ were generally similar to those of the corresponding 16S rRNA genes. Although some genomes contained multiple copies of narG, recent horizontal gene transfer of narG was not apparent.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Environmental Science(all)
- Ecology
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In: Applied and Environmental Microbiology, Vol. 75, No. 15, 08.2009, p. 5170-5174.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genome-derived criteria for assigning environmental narG and nosZ sequences to operational taxonomic units of nitrate reducers
AU - Palmer, Katharina
AU - Drake, Harold L.
AU - Horn, Marcus A.
N1 - Copyright: Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/8
Y1 - 2009/8
N2 - Ninety percent of cultured bacterial nitrate reducers with a 16S rRNA gene similarity of ≥97% had a narG or nosZ similarity of ≥67% or ≥80%, respectively, suggesting that 67% and 80% could be used as standardized, conservative threshold similarity values for narG and nosZ, respectively (i.e., any two sequences that are less similar than the threshold similarity value have a very high probability of belonging to different species), for estimating species-level operational taxonomic units. Genus-level tree topologies of narG and nosZ were generally similar to those of the corresponding 16S rRNA genes. Although some genomes contained multiple copies of narG, recent horizontal gene transfer of narG was not apparent.
AB - Ninety percent of cultured bacterial nitrate reducers with a 16S rRNA gene similarity of ≥97% had a narG or nosZ similarity of ≥67% or ≥80%, respectively, suggesting that 67% and 80% could be used as standardized, conservative threshold similarity values for narG and nosZ, respectively (i.e., any two sequences that are less similar than the threshold similarity value have a very high probability of belonging to different species), for estimating species-level operational taxonomic units. Genus-level tree topologies of narG and nosZ were generally similar to those of the corresponding 16S rRNA genes. Although some genomes contained multiple copies of narG, recent horizontal gene transfer of narG was not apparent.
UR - http://www.scopus.com/inward/record.url?scp=67651250329&partnerID=8YFLogxK
U2 - 10.1128/AEM.00254-09
DO - 10.1128/AEM.00254-09
M3 - Article
C2 - 19502444
AN - SCOPUS:67651250329
VL - 75
SP - 5170
EP - 5174
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 15
ER -