Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Publisher | International Society for Horticultural Science |
| Pages | 75-80 |
| Number of pages | 6 |
| ISBN (print) | 9789066056923 |
| Publication status | Published - 2009 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 847 |
| ISSN (Print) | 0567-7572 |
Abstract
Genetic modification has proved to be an effective way in controlling ethylene synthesis and perception. Use of the mutant ethylene receptor gene, etr1-1, from Arabidopsis seems most promising, especially when it is expressed under the control of a flower specific promoter. In order to introduce the etr1-1 mutant gene in ornamental plant species we established effective regeneration and transformation systems for a range of popular potted plants including Campanula, Kalanchoë, Rhipsalidopsis and two orchid species Oncidium and Odontoglossum. Fertile transgenic adult plants of Campanula carpatica Jacq. and Kalanchoë blossfeldiana Poeln. were obtained by Agrobacterium tumefaciens-mediated transformation. The construct used for transformation contained etr1-1 gene under the control of the petal specific fbp1-promoter from petunia. Flowering T0 lines were tested for their ethylene sensitivity in 2 μl L-1 ethylene. The flowers on control (non transgenic) Kalanchoë and Campanula plants wilted within 2 and 3 days, respectively. The best transgenic line of Campanula flowered up to 27 days in ethylene, while the best Kalanchoë line had most of the flowers open for longer than 10 days of continuous ethylene exposure. T1 progenies of both species showed stable inheritance and expression of etr1-1, which make them useful for future breeding. Before the ethylene insensitive plants can be commercially available, several issues have to be solved. Use of nptII for selection may cause implication by registration for commercial use, especially in European countries. Furthermore, molecular analysis indicated unwished expression of etr1-1 in leaves or roots, which in some cases correlated with lower ability of rooting of cuttings. Use of flower specific promoters from the species of interest can be recommended to solve the problem.
Keywords
- Agrobacterium tumefaciens, Campanula, Etr1-1, Flower longevity, Flower specific promoter - fbp1, Inheritance, Kalanchoë, Odontoglossum, Oncidium, Rhipsalidopsis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. International Society for Horticultural Science, 2009. p. 75-80 (Acta Horticulturae; Vol. 847).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Genetically modified Flowering potted plants with reduced ethylene sensitivity
AU - Mibus, H.
AU - Sriskandarajah, S.
AU - Serek, M.
PY - 2009
Y1 - 2009
N2 - Genetic modification has proved to be an effective way in controlling ethylene synthesis and perception. Use of the mutant ethylene receptor gene, etr1-1, from Arabidopsis seems most promising, especially when it is expressed under the control of a flower specific promoter. In order to introduce the etr1-1 mutant gene in ornamental plant species we established effective regeneration and transformation systems for a range of popular potted plants including Campanula, Kalanchoë, Rhipsalidopsis and two orchid species Oncidium and Odontoglossum. Fertile transgenic adult plants of Campanula carpatica Jacq. and Kalanchoë blossfeldiana Poeln. were obtained by Agrobacterium tumefaciens-mediated transformation. The construct used for transformation contained etr1-1 gene under the control of the petal specific fbp1-promoter from petunia. Flowering T0 lines were tested for their ethylene sensitivity in 2 μl L-1 ethylene. The flowers on control (non transgenic) Kalanchoë and Campanula plants wilted within 2 and 3 days, respectively. The best transgenic line of Campanula flowered up to 27 days in ethylene, while the best Kalanchoë line had most of the flowers open for longer than 10 days of continuous ethylene exposure. T1 progenies of both species showed stable inheritance and expression of etr1-1, which make them useful for future breeding. Before the ethylene insensitive plants can be commercially available, several issues have to be solved. Use of nptII for selection may cause implication by registration for commercial use, especially in European countries. Furthermore, molecular analysis indicated unwished expression of etr1-1 in leaves or roots, which in some cases correlated with lower ability of rooting of cuttings. Use of flower specific promoters from the species of interest can be recommended to solve the problem.
AB - Genetic modification has proved to be an effective way in controlling ethylene synthesis and perception. Use of the mutant ethylene receptor gene, etr1-1, from Arabidopsis seems most promising, especially when it is expressed under the control of a flower specific promoter. In order to introduce the etr1-1 mutant gene in ornamental plant species we established effective regeneration and transformation systems for a range of popular potted plants including Campanula, Kalanchoë, Rhipsalidopsis and two orchid species Oncidium and Odontoglossum. Fertile transgenic adult plants of Campanula carpatica Jacq. and Kalanchoë blossfeldiana Poeln. were obtained by Agrobacterium tumefaciens-mediated transformation. The construct used for transformation contained etr1-1 gene under the control of the petal specific fbp1-promoter from petunia. Flowering T0 lines were tested for their ethylene sensitivity in 2 μl L-1 ethylene. The flowers on control (non transgenic) Kalanchoë and Campanula plants wilted within 2 and 3 days, respectively. The best transgenic line of Campanula flowered up to 27 days in ethylene, while the best Kalanchoë line had most of the flowers open for longer than 10 days of continuous ethylene exposure. T1 progenies of both species showed stable inheritance and expression of etr1-1, which make them useful for future breeding. Before the ethylene insensitive plants can be commercially available, several issues have to be solved. Use of nptII for selection may cause implication by registration for commercial use, especially in European countries. Furthermore, molecular analysis indicated unwished expression of etr1-1 in leaves or roots, which in some cases correlated with lower ability of rooting of cuttings. Use of flower specific promoters from the species of interest can be recommended to solve the problem.
KW - Agrobacterium tumefaciens
KW - Campanula
KW - Etr1-1
KW - Flower longevity
KW - Flower specific promoter - fbp1
KW - Inheritance
KW - Kalanchoë
KW - Odontoglossum
KW - Oncidium
KW - Rhipsalidopsis
UR - http://www.scopus.com/inward/record.url?scp=75649120381&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2009.847.8
DO - 10.17660/ActaHortic.2009.847.8
M3 - Conference contribution
AN - SCOPUS:75649120381
SN - 9789066056923
T3 - Acta Horticulturae
SP - 75
EP - 80
BT - Acta Horticulturae
PB - International Society for Horticultural Science
ER -