Details
| Original language | English |
|---|---|
| Pages (from-to) | 597-604 |
| Number of pages | 8 |
| Journal | Biotechnology and Biotechnological Equipment |
| Volume | 33 |
| Issue number | 1 |
| Early online date | 9 Apr 2019 |
| Publication status | E-pub ahead of print - 9 Apr 2019 |
Abstract
We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.
Keywords
- AGAMOUS, number of petals, Rosa x damascena Mill f. trigintipetala
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology and Biotechnological Equipment, Vol. 33, No. 1, 09.04.2019, p. 597-604.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genetic control of flower petal number in Rosa x Damascena Mill f. trigintipetala
AU - Rusanov, Krasimir
AU - Kovacheva, Natasha
AU - Rusanova, Mila
AU - Linde, Marcus
AU - Debener, Thomas
AU - Atanassov, Ivan
N1 - Funding Information: This study was supported by the National Science Fund at the Ministry of Education and Science, Bulgaria, under grant DN 06/2. The authors would like to thank Marina Alexeeva (Master degree student at the Sofia University) and Rumyana Velcheva (technician at ABI) for the excellent technical assistance.
PY - 2019/4/9
Y1 - 2019/4/9
N2 - We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.
AB - We studied the petal number trait in a population obtained after self-pollination of R. x damascena f. trigintipetala following analysis of molecular markers which have previously been mapped near the major dominant locus Blfo/d6 determining this trait in other rose species including R. multiflora and R. hybrida. The results showed that the same genetic mechanism, which determines the petal number trait in R. multiflora and R. hybrida also controls the trait in R. x damascena f. trigintipetala and is related to the dominant effect of a single copy allele in the tetraploid genome of this species. We also analyzed the expression of several flower homeotic genes including R. x damascena APETALA1/FUL-like (paleo AP1 type), R. x damascena euAPETALA 3 (euAP3 line) and R. x damascena AGAMOUS in early stage flower buds corresponding to plants with double and simple flowers. The obtained results showed that only R. x damascena AGAMOUS was differentially expressed between the samples of double and simple flowers, its relative expression being upregulated 3.5-fold in simple flowers. We further cloned and sequenced the four genomic clones of R. x damascena AGAMOUS and studied the potential additive effect of this gene by analysing the segregation of its four alleles in the population of self-pollinated R. x damascena. Analysis of variance of the data for petal number and allele segregation did not show a statistically significant effect of any allele configuration of the AGAMOUS gene on the petal number trait in R. x damascena f. trigintipetala.
KW - AGAMOUS
KW - number of petals
KW - Rosa x damascena Mill f. trigintipetala
UR - http://www.scopus.com/inward/record.url?scp=85064155743&partnerID=8YFLogxK
U2 - 10.1080/13102818.2019.1599731
DO - 10.1080/13102818.2019.1599731
M3 - Article
AN - SCOPUS:85064155743
VL - 33
SP - 597
EP - 604
JO - Biotechnology and Biotechnological Equipment
JF - Biotechnology and Biotechnological Equipment
SN - 1310-2818
IS - 1
ER -