Details
| Original language | English |
|---|---|
| Article number | 109277 |
| Journal | Scientia horticulturae |
| Volume | 266 |
| Early online date | 21 Feb 2020 |
| Publication status | Published - 10 May 2020 |
Abstract
In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.
Keywords
- Adventitious root induction, Association mapping, Auxin, In vitro rooting, Marker, Marker-trait-association, Rosa, SNP
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Scientia horticulturae, Vol. 266, 109277, 10.05.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genetic analysis of adventitious root formation in vivo and in vitro in a diversity panel of roses
AU - Nguyen, Thi Hong Nhung
AU - Tänzer, Sophie
AU - Rudeck, Jasmin
AU - Winkelmann, Traud
AU - Debener, Thomas
N1 - Funding information: This study was carried out with financial support from the Vietnamese Government and the unit of Molecular Plant Breeding, Institute of Plant Genetics, Leibniz University Hannover, Germany. We would like to thank Klaus Dreier and his team from the department of Molecular Plant Breeding for assistance in the greenhouse experiment. This study was carried out with financial support from the Vietnamese Government and the unit of Molecular Plant Breeding , Institute of Plant Genetics , Leibniz University Hannover , Germany. We would like to thank Klaus Dreier and his team from the department of Molecular Plant Breeding for assistance in the greenhouse experiment.
PY - 2020/5/10
Y1 - 2020/5/10
N2 - In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.
AB - In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.
KW - Adventitious root induction
KW - Association mapping
KW - Auxin
KW - In vitro rooting
KW - Marker
KW - Marker-trait-association
KW - Rosa
KW - SNP
UR - http://www.scopus.com/inward/record.url?scp=85079593767&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2020.109277
DO - 10.1016/j.scienta.2020.109277
M3 - Article
AN - SCOPUS:85079593767
VL - 266
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
M1 - 109277
ER -