TY - JOUR

T1 - Genetic analysis of adventitious root formation in vivo and in vitro in a diversity panel of roses

AU - Nguyen, Thi Hong Nhung

AU - Tänzer, Sophie

AU - Rudeck, Jasmin

AU - Winkelmann, Traud

AU - Debener, Thomas

N1 - Funding information: This study was carried out with financial support from the Vietnamese Government and the unit of Molecular Plant Breeding, Institute of Plant Genetics, Leibniz University Hannover, Germany. We would like to thank Klaus Dreier and his team from the department of Molecular Plant Breeding for assistance in the greenhouse experiment. This study was carried out with financial support from the Vietnamese Government and the unit of Molecular Plant Breeding , Institute of Plant Genetics , Leibniz University Hannover , Germany. We would like to thank Klaus Dreier and his team from the department of Molecular Plant Breeding for assistance in the greenhouse experiment.

PY - 2020/5/10

Y1 - 2020/5/10

N2 - In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.

AB - In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.

KW - Adventitious root induction

KW - Association mapping

KW - Auxin

KW - In vitro rooting

KW - Marker

KW - Marker-trait-association

KW - Rosa

KW - SNP

UR - http://www.scopus.com/inward/record.url?scp=85079593767&partnerID=8YFLogxK

U2 - 10.1016/j.scienta.2020.109277

DO - 10.1016/j.scienta.2020.109277

M3 - Article

AN - SCOPUS:85079593767

VL - 266

JO - Scientia horticulturae

JF - Scientia horticulturae

SN - 0304-4238

M1 - 109277

ER -