TY - JOUR

T1 - Fungi and bacteria respond differently to changing environmental conditions within a soil profile

AU - Preusser, Sebastian

AU - Poll, Christian

AU - Marhan, Sven

AU - Angst, Gerrit

AU - Mueller, Carsten W.

AU - Bachmann, Jörg

AU - Kandeler, Ellen

N1 - Funding Information: We would like to thank Stefanie Heinze, Nils Borchard and Bernd Marschner for the project coordination, Sabine Rudolph and Heike Haslwimmer for their assistance in the laboratory, Wolfgang Armbruster at Institute of Food Chemistry at University of Hohenheim for IRMS measurements, Kathleen Regan for English corrections, and Mathias Bischoff and Andreas Lieb for their help during the soil samplings. This project was part of the Research Unit “The Forgotten Part of Carbon Cycling: Organic Matter Storage and Turnover in Subsoils (SUBSOM)” and funded by the Deutsche Forschungsgemeinschaft DFG ( FOR1806 , KA 1590/11-1 , KA 1590/11-2 , MA 4436/3-2 , PO 1578/4-2 ).

PY - 2019/10

Y1 - 2019/10

N2 - Contrasting environmental conditions in topsoil and subsoil determine both abundance and function of soil microbial communities, affecting carbon (C) dynamics throughout the entire soil profile. Although the response of soil microorganisms to single factors such as substrate availability or micro-climatic conditions has been frequently studied, fewer studies have focused on complex interactions between substrate availability and environmental conditions. To address this, we employed vertical soil translocations between topsoil and subsoil horizons of an acid and sandy Dystric Cambisol under European beech forest in Lower Saxony, Germany, to investigate the impact of changing habitat conditions on microbial decomposer communities. To follow microbial substrate utilization at different soil depths, we created hot spots of fresh organic matter (OM) by adding 13C-labelled root litter. Soil samples were taken every three months over an experimental period of twelve months (June 2014 to June 2015). Generally, microbial biomass was strongly controlled by C availability throughout the profile. The importance of root litter as a microbial C source increased from topsoil to subsoil, but changes in available C sources affected fungi and bacteria differently. Fungi preferentially used root litter-derived C throughout the entire soil profile, demonstrating that limited access to preferred substrates, rather than micro-climatic conditions, was the main driver of decreasing fungal abundance with soil depth. In contrast, bacteria intensified utilization of root-derived C only in the absence of alternative C sources in the subsoil and were more strongly affected by spatial separation from C sources. Low soil moisture in combination with the highly sandy subsoil environment limited bacterial access to their substrates and, consequently, bacterial growth. In conclusion, fungal C utilization relies mainly on the quantity of recent plant-derived substrates, whereas bacterial access to substrates is additionally controlled by environmental conditions. This study indicates that limited microbial access to their heterogeneously distributed substrates may be an important factor for C accumulation and stabilization in subsoils.

AB - Contrasting environmental conditions in topsoil and subsoil determine both abundance and function of soil microbial communities, affecting carbon (C) dynamics throughout the entire soil profile. Although the response of soil microorganisms to single factors such as substrate availability or micro-climatic conditions has been frequently studied, fewer studies have focused on complex interactions between substrate availability and environmental conditions. To address this, we employed vertical soil translocations between topsoil and subsoil horizons of an acid and sandy Dystric Cambisol under European beech forest in Lower Saxony, Germany, to investigate the impact of changing habitat conditions on microbial decomposer communities. To follow microbial substrate utilization at different soil depths, we created hot spots of fresh organic matter (OM) by adding 13C-labelled root litter. Soil samples were taken every three months over an experimental period of twelve months (June 2014 to June 2015). Generally, microbial biomass was strongly controlled by C availability throughout the profile. The importance of root litter as a microbial C source increased from topsoil to subsoil, but changes in available C sources affected fungi and bacteria differently. Fungi preferentially used root litter-derived C throughout the entire soil profile, demonstrating that limited access to preferred substrates, rather than micro-climatic conditions, was the main driver of decreasing fungal abundance with soil depth. In contrast, bacteria intensified utilization of root-derived C only in the absence of alternative C sources in the subsoil and were more strongly affected by spatial separation from C sources. Low soil moisture in combination with the highly sandy subsoil environment limited bacterial access to their substrates and, consequently, bacterial growth. In conclusion, fungal C utilization relies mainly on the quantity of recent plant-derived substrates, whereas bacterial access to substrates is additionally controlled by environmental conditions. This study indicates that limited microbial access to their heterogeneously distributed substrates may be an important factor for C accumulation and stabilization in subsoils.

KW - Carbon cycle

KW - Detritusphere

KW - Habitat conditions

KW - Soil microorganisms

KW - Stable isotopes

KW - Subsoil

UR - http://www.scopus.com/inward/record.url?scp=85070190205&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2019.107543

DO - 10.1016/j.soilbio.2019.107543

M3 - Article

AN - SCOPUS:85070190205

VL - 137

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 107543

ER -