Details
| Original language | English |
|---|---|
| Pages (from-to) | 1055-1066 |
| Number of pages | 12 |
| Journal | Biomedical microdevices |
| Volume | 15 |
| Issue number | 6 |
| Publication status | Published - 28 Jul 2013 |
Abstract
Bone tissue engineering is an alternative approach to bone grafts. In our study we aim to develop a composite scaffold for bone regeneration made of doped zirconium oxide (ZrO2) conjugated with poly(lactic-co-glycolic acid) (PLGA) particles for the delivery of growth factors. In this composite, the PLGA microspheres are designed to release a crucial growth factor for bone formation, bone morphogenetic protein-2 (BMP2). We found that by changing the polymer's molecular weight and composition, we could control microsphere loading, release and size. The BMP2 released from PLGA microspheres retained its biological activity and increased osteoblastic marker expression in human mesenchymal stem cells (hMSCs). Uncapped PLGA microspheres were conjugated to ZrO2 scaffolds using carbodiimide chemistry, and the composite scaffold was shown to support hMSCs growth. We also demonstrated that human umbilical vein endothelial cells (HUVECs) can be co-cultured with hMSCs on the ZrO2 scaffold for future vascularization of the scaffold. The ZrO2 composite scaffold could serve as a bone substitute for bone grafting applications with the added ability of releasing different growth factors needed for bone regeneration.
Keywords
- BMP2, Bone tissue engineering, Drug delivery, PLGA, Scaffold
ASJC Scopus subject areas
- Engineering(all)
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Biomedical microdevices, Vol. 15, No. 6, 28.07.2013, p. 1055-1066.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Functionalized PLGA-doped zirconium oxide ceramics for bone tissue regeneration
AU - Lupu-Haber, Yael
AU - Pinkas, Oded
AU - Boehm, Stefanie
AU - Scheper, Thomas
AU - Kasper, Cornelia
AU - Machluf, Marcelle
N1 - Funding information: Acknowledgments The research was supported by the Niedersachsen Foundation. The partial support of the Russell Berrie Nanotechnology Institute, Technion–Israel Institute of Technology (IIT), is thankfully acknowledged. bFGF was kindly donated by Prof. Gera Neufeld, from the Department of Anatomy and Cell Biology, Faculty of Medicine, IIT. BMP2 was kindly donated by Prof. Walter Sebald, from Biozentrum University, Würzburg, Germany. We further wish to thank Ilana Schoenbrun for her technical assistance.
PY - 2013/7/28
Y1 - 2013/7/28
N2 - Bone tissue engineering is an alternative approach to bone grafts. In our study we aim to develop a composite scaffold for bone regeneration made of doped zirconium oxide (ZrO2) conjugated with poly(lactic-co-glycolic acid) (PLGA) particles for the delivery of growth factors. In this composite, the PLGA microspheres are designed to release a crucial growth factor for bone formation, bone morphogenetic protein-2 (BMP2). We found that by changing the polymer's molecular weight and composition, we could control microsphere loading, release and size. The BMP2 released from PLGA microspheres retained its biological activity and increased osteoblastic marker expression in human mesenchymal stem cells (hMSCs). Uncapped PLGA microspheres were conjugated to ZrO2 scaffolds using carbodiimide chemistry, and the composite scaffold was shown to support hMSCs growth. We also demonstrated that human umbilical vein endothelial cells (HUVECs) can be co-cultured with hMSCs on the ZrO2 scaffold for future vascularization of the scaffold. The ZrO2 composite scaffold could serve as a bone substitute for bone grafting applications with the added ability of releasing different growth factors needed for bone regeneration.
AB - Bone tissue engineering is an alternative approach to bone grafts. In our study we aim to develop a composite scaffold for bone regeneration made of doped zirconium oxide (ZrO2) conjugated with poly(lactic-co-glycolic acid) (PLGA) particles for the delivery of growth factors. In this composite, the PLGA microspheres are designed to release a crucial growth factor for bone formation, bone morphogenetic protein-2 (BMP2). We found that by changing the polymer's molecular weight and composition, we could control microsphere loading, release and size. The BMP2 released from PLGA microspheres retained its biological activity and increased osteoblastic marker expression in human mesenchymal stem cells (hMSCs). Uncapped PLGA microspheres were conjugated to ZrO2 scaffolds using carbodiimide chemistry, and the composite scaffold was shown to support hMSCs growth. We also demonstrated that human umbilical vein endothelial cells (HUVECs) can be co-cultured with hMSCs on the ZrO2 scaffold for future vascularization of the scaffold. The ZrO2 composite scaffold could serve as a bone substitute for bone grafting applications with the added ability of releasing different growth factors needed for bone regeneration.
KW - BMP2
KW - Bone tissue engineering
KW - Drug delivery
KW - PLGA
KW - Scaffold
UR - http://www.scopus.com/inward/record.url?scp=84890570604&partnerID=8YFLogxK
U2 - 10.1007/s10544-013-9797-1
DO - 10.1007/s10544-013-9797-1
M3 - Article
C2 - 23893013
AN - SCOPUS:84890570604
VL - 15
SP - 1055
EP - 1066
JO - Biomedical microdevices
JF - Biomedical microdevices
SN - 1387-2176
IS - 6
ER -