Details
| Original language | English |
|---|---|
| Article number | 4397 |
| Number of pages | 10 |
| Journal | Scientific reports |
| Volume | 12 |
| Issue number | 1 |
| Early online date | 15 Mar 2022 |
| Publication status | Published - Dec 2022 |
Abstract
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
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In: Scientific reports, Vol. 12, No. 1, 4397, 12.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection
AU - Könemund, Lea
AU - Neumann, Laurie
AU - Hirschberg, Felix
AU - Biedendieck, Rebekka
AU - Jahn, Dieter
AU - Johannes, Hans Hermann
AU - Kowalsky, Wolfgang
N1 - Funding Information: The authors gratefully acknowledge the Lower Saxony Ministry for Science and Culture (MWK) for funding the Quantum- and Nanometrology (QUANOMET) Project Number ZN3294 at the Laboratory for Emerging Nanometrology (LENA), DFG Cluster of Excellence PhoenixD (Exc 2122, Project ID 390833453), and particularly the Volkswagen Foundation for funding Project Laboratory Across Borders (ProLAB) Project Number 96620. We further express our gratitude to the Publication Fund of the Technische Universität Braunschweig. Open Access funding enabled and organized by Projekt DEAL
PY - 2022/12
Y1 - 2022/12
N2 - Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
AB - Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
UR - http://www.scopus.com/inward/record.url?scp=85126682948&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-08272-3
DO - 10.1038/s41598-022-08272-3
M3 - Article
C2 - 35292706
AN - SCOPUS:85126682948
VL - 12
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 4397
ER -