Details
| Original language | English |
|---|---|
| Pages (from-to) | 35797-35802 |
| Number of pages | 6 |
| Journal | RSC Advances |
| Volume | 9 |
| Issue number | 61 |
| Early online date | 4 Nov 2019 |
| Publication status | Published - 2019 |
Abstract
Ectopic expression of BC1 which encodes a putative pathway specific transcription factor from the ACE1 biosynthetic gene cluster of the rice pathogen Pyricularia oryzae Guy11 did not lead to the production of ACE1-related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of pyiR which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in Magnaporthe grisea NI980 led to the apparent up-regulation of the pyi cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Chemical Engineering(all)
- General Chemical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: RSC Advances, Vol. 9, No. 61, 2019, p. 35797-35802.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Function of pathway specific regulators in the ACE1 and pyrichalasin H biosynthetic gene clusters
AU - Hantke, Verena
AU - Wang, Chongqing
AU - Skellam, Elizabeth J.
AU - Cox, Russell J.
N1 - Funding information: DFG is thanked for the provision of NMR and LCMS equipment (INST 187/621-1, INST 187/686-1). VH was funded by DFG (CO 1328/2-1) and CW was funded by the China Scholarship Council (2016 08310143). Dr Colin Lazarus, University of Bristol, is thanked for the gi?s of pTHGS-eGFP and pTYGS-ade. The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2019
Y1 - 2019
N2 - Ectopic expression of BC1 which encodes a putative pathway specific transcription factor from the ACE1 biosynthetic gene cluster of the rice pathogen Pyricularia oryzae Guy11 did not lead to the production of ACE1-related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of pyiR which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in Magnaporthe grisea NI980 led to the apparent up-regulation of the pyi cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.
AB - Ectopic expression of BC1 which encodes a putative pathway specific transcription factor from the ACE1 biosynthetic gene cluster of the rice pathogen Pyricularia oryzae Guy11 did not lead to the production of ACE1-related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of pyiR which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in Magnaporthe grisea NI980 led to the apparent up-regulation of the pyi cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.
UR - http://www.scopus.com/inward/record.url?scp=85074850985&partnerID=8YFLogxK
U2 - 10.1039/c9ra07028a
DO - 10.1039/c9ra07028a
M3 - Article
AN - SCOPUS:85074850985
VL - 9
SP - 35797
EP - 35802
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 61
ER -