Details
| Original language | English |
|---|---|
| Title of host publication | Frontiers in Ultrafast Optics |
| Subtitle of host publication | Biomedical, Scientific, and Industrial Applications X |
| Publication status | Published - 25 Feb 2010 |
| Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X - San Francisco, CA, United States Duration: 24 Jan 2010 → 26 Jan 2010 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 7589 |
| ISSN (Print) | 0277-786X |
Abstract
The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.
Keywords
- Biophotonics, Cell manipulation, GFP, Membrane permeabilization, Nanoparticles, Nanorods, Perforation, Plasmonics, Transfection, Ultrashort laser pulses
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
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- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X. 2010. 75890C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7589).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Fs-laser cell perforation using gold nanoparticles of different shapes
AU - Schomaker, Markus
AU - Fehlauer, Holger
AU - Bintig, Willem
AU - Ngezahayo, Anaclet
AU - Nolte, Ingo
AU - Murua Escobar, Hugo
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2010/2/25
Y1 - 2010/2/25
N2 - The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.
AB - The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.
KW - Biophotonics
KW - Cell manipulation
KW - GFP
KW - Membrane permeabilization
KW - Nanoparticles
KW - Nanorods
KW - Perforation
KW - Plasmonics
KW - Transfection
KW - Ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=77951694419&partnerID=8YFLogxK
U2 - 10.1117/12.842446
DO - 10.1117/12.842446
M3 - Conference contribution
AN - SCOPUS:77951694419
SN - 9780819479853
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X
Y2 - 24 January 2010 through 26 January 2010
ER -