Details
| Original language | English |
|---|---|
| Pages (from-to) | 175-191 |
| Number of pages | 17 |
| Journal | Journal of Biomaterials Applications |
| Volume | 24 |
| Issue number | 2 |
| Publication status | Published - 1 Aug 2009 |
Abstract
The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit® II which is coated with a silica-nanostructure or with plain Bioverit® II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit® II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.
Keywords
- Biocompatibility, Bioverit® II, Histology, Middle ear, Mouse, Silica-nanocoating
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
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In: Journal of Biomaterials Applications, Vol. 24, No. 2, 01.08.2009, p. 175-191.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Free Bioverit® II implants coated with a nanoporous silica layer in a mouse ear model
T2 - A histological study
AU - Vogt, Julia C.
AU - Brandes, Gudrun
AU - Ehlert, Nina
AU - Behrens, Peter
AU - Nolte, Ingo
AU - Mueller, Peter P.
AU - Lenarz, Thomas
AU - Stieve, Martin
PY - 2009/8/1
Y1 - 2009/8/1
N2 - The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit® II which is coated with a silica-nanostructure or with plain Bioverit® II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit® II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.
AB - The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit® II which is coated with a silica-nanostructure or with plain Bioverit® II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit® II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.
KW - Biocompatibility
KW - Bioverit® II
KW - Histology
KW - Middle ear
KW - Mouse
KW - Silica-nanocoating
UR - http://www.scopus.com/inward/record.url?scp=70149099973&partnerID=8YFLogxK
U2 - 10.1177/0885328208095469
DO - 10.1177/0885328208095469
M3 - Article
C2 - 18987019
AN - SCOPUS:70149099973
VL - 24
SP - 175
EP - 191
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
SN - 0885-3282
IS - 2
ER -