Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy

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  • Universidad de Costa Rica
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Original languageEnglish
Title of host publicationCONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers
Pages5-9
Number of pages5
Publication statusPublished - 11 Apr 2011
Event21st International Conference on Electronics Communications and Computers, CONIELECOMP 2011 - Cholula, Mexico
Duration: 28 Feb 20112 Mar 2011

Abstract

In this paper, an algorithm is introduced for segmenting the foreground regions present in a human insulin crystal intensity image captured by an in-situ microscope inside of a bioreactor. The segmentation is carried out by classifying all image pixels into pixels belonging to the foreground regions and pixels belonging to the background region. For classification, the local intensity variance at each pixel position is compared to a threshold. Those pixels whose local intensity variance is bigger than the threshold are classified as belonging to the foreground regions. The threshold is estimated as a linear combination of two statistical characteristics of the local intensity variance values at the pixels in the background region. Those statistical characteristics are estimated from the histogram of the local intensity variance values of all image pixels by maximizing a likelihood function using an Expectation and Maximization approach. Misclassifications are corrected by particle filtering. Experimental results on real data revealed a processing time of 11.82 seconds/image, an excellent reliability and a segmentation error of approximately 14 pixels.

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Cite this

Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy. / Martinez, Geovanni; Lindner, Patrick; Scheper, Thomas.
CONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers. 2011. p. 5-9.

Research output: Chapter in book/report/conference proceedingConference contributionResearchpeer review

Martinez, G, Lindner, P & Scheper, T 2011, Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy. in CONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers. pp. 5-9, 21st International Conference on Electronics Communications and Computers, CONIELECOMP 2011, Cholula, Mexico, 28 Feb 2011. https://doi.org/10.1109/CONIELECOMP.2011.5749322
Martinez, G., Lindner, P., & Scheper, T. (2011). Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy. In CONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers (pp. 5-9) https://doi.org/10.1109/CONIELECOMP.2011.5749322
Martinez G, Lindner P, Scheper T. Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy. In CONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers. 2011. p. 5-9 doi: 10.1109/CONIELECOMP.2011.5749322
Martinez, Geovanni ; Lindner, Patrick ; Scheper, Thomas. / Foreground Segmentation of Human Insulin Crystal Images for In-Situ Microscopy. CONIELECOMP 2011, 21st International Conference on Electrical Communications and Computers. 2011. pp. 5-9
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