Details
| Original language | English |
|---|---|
| Pages (from-to) | 125-131 |
| Number of pages | 7 |
| Journal | Journal of biotechnology |
| Volume | 152 |
| Issue number | 4 |
| Publication status | Published - 27 Jul 2010 |
Abstract
Efficient techniques for the isolation of enzymes from a microbial production culture are required to meet the growing needs of the " White Biotechnologies" for novel catalysts. Traditional protein purification procedures typically comprise multistep operations, which inevitably come along with significant losses of enzyme activity. Foaming offers an alternative minimizing the processing steps, preserving the purification efficiency and decreasing the activity losses all at the same time. This review provides an insight into the foaming process itself and its application in separating enzymes from model systems and from complex media, such as microbial cultures. Examples demonstrate fractionated foaming and the tweezer technique.
Keywords
- Enzymes, Foam fractionation, Proteins, Purification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 152, No. 4, 27.07.2010, p. 125-131.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Foaming of proteins
T2 - New prospects for enzyme purification processes
AU - Linke, D.
AU - Berger, R. G.
PY - 2010/7/27
Y1 - 2010/7/27
N2 - Efficient techniques for the isolation of enzymes from a microbial production culture are required to meet the growing needs of the " White Biotechnologies" for novel catalysts. Traditional protein purification procedures typically comprise multistep operations, which inevitably come along with significant losses of enzyme activity. Foaming offers an alternative minimizing the processing steps, preserving the purification efficiency and decreasing the activity losses all at the same time. This review provides an insight into the foaming process itself and its application in separating enzymes from model systems and from complex media, such as microbial cultures. Examples demonstrate fractionated foaming and the tweezer technique.
AB - Efficient techniques for the isolation of enzymes from a microbial production culture are required to meet the growing needs of the " White Biotechnologies" for novel catalysts. Traditional protein purification procedures typically comprise multistep operations, which inevitably come along with significant losses of enzyme activity. Foaming offers an alternative minimizing the processing steps, preserving the purification efficiency and decreasing the activity losses all at the same time. This review provides an insight into the foaming process itself and its application in separating enzymes from model systems and from complex media, such as microbial cultures. Examples demonstrate fractionated foaming and the tweezer technique.
KW - Enzymes
KW - Foam fractionation
KW - Proteins
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=79955138236&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2010.07.022
DO - 10.1016/j.jbiotec.2010.07.022
M3 - Review article
C2 - 20670663
AN - SCOPUS:79955138236
VL - 152
SP - 125
EP - 131
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 4
ER -