Details
| Original language | English |
|---|---|
| Pages (from-to) | 323-327 |
| Number of pages | 5 |
| Journal | LIPIDS |
| Volume | 40 |
| Issue number | 3 |
| Publication status | Published - 1 Mar 2005 |
Abstract
Active Upases were Isolated from the culture supernatant of the basldlomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N 2 min-1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus, The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: LIPIDS, Vol. 40, No. 3, 01.03.2005, p. 323-327.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus)
AU - Linke, Diana
AU - Zorn, Holger
AU - Gerken, Birte
AU - Parlar, Harun
AU - Berger, Ralf G.
N1 - Funding information: Financial support by the Arbeitsgemeinschaft Industrieller Forschungs vereinigungen e.V. (project #121 ZN) through the Forschungskreis der Ernährungsindustrie e.V. (Bonn, Germany) is gratefully acknowledged. The project is part of the joint initiative “Biologisch aktive Naturstoffe—Chemische Diversität” at the University of Hannover.
PY - 2005/3/1
Y1 - 2005/3/1
N2 - Active Upases were Isolated from the culture supernatant of the basldlomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N 2 min-1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus, The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
AB - Active Upases were Isolated from the culture supernatant of the basldlomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N 2 min-1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus, The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
UR - http://www.scopus.com/inward/record.url?scp=18344386258&partnerID=8YFLogxK
U2 - 10.1007/s11745-005-1389-x
DO - 10.1007/s11745-005-1389-x
M3 - Article
C2 - 15957260
AN - SCOPUS:18344386258
VL - 40
SP - 323
EP - 327
JO - LIPIDS
JF - LIPIDS
SN - 0024-4201
IS - 3
ER -