Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus)

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Diana Linke
  • Holger Zorn
  • Birte Gerken
  • Harun Parlar
  • Ralf G. Berger

Research Organisations

External Research Organisations

  • Technical University of Munich (TUM)
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Details

Original languageEnglish
Pages (from-to)323-327
Number of pages5
JournalLIPIDS
Volume40
Issue number3
Publication statusPublished - 1 Mar 2005

Abstract

Active Upases were Isolated from the culture supernatant of the basldlomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N 2 min-1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus, The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.

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Cite this

Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus). / Linke, Diana; Zorn, Holger; Gerken, Birte et al.
In: LIPIDS, Vol. 40, No. 3, 01.03.2005, p. 323-327.

Research output: Contribution to journalArticleResearchpeer review

Linke, D, Zorn, H, Gerken, B, Parlar, H & Berger, RG 2005, 'Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus)', LIPIDS, vol. 40, no. 3, pp. 323-327. https://doi.org/10.1007/s11745-005-1389-x
Linke, D., Zorn, H., Gerken, B., Parlar, H., & Berger, R. G. (2005). Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus). LIPIDS, 40(3), 323-327. https://doi.org/10.1007/s11745-005-1389-x
Linke D, Zorn H, Gerken B, Parlar H, Berger RG. Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus). LIPIDS. 2005 Mar 1;40(3):323-327. doi: 10.1007/s11745-005-1389-x
Linke, Diana ; Zorn, Holger ; Gerken, Birte et al. / Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus). In: LIPIDS. 2005 ; Vol. 40, No. 3. pp. 323-327.
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AU - Zorn, Holger

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AU - Parlar, Harun

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