Details
| Original language | English |
|---|---|
| Pages (from-to) | 653-657 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 42 |
| Issue number | 5 |
| Publication status | Published - Jan 1995 |
| Externally published | Yes |
Abstract
Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 42, No. 5, 01.1995, p. 653-657.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Fluorometric measurement of poly-β hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry
AU - Degelau, A.
AU - Scheper, T.
AU - Bailey, J. E.
AU - Guske, C.
PY - 1995/1
Y1 - 1995/1
N2 - Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
AB - Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.
UR - http://www.scopus.com/inward/record.url?scp=0028984113&partnerID=8YFLogxK
U2 - 10.1007/BF00171939
DO - 10.1007/BF00171939
M3 - Article
AN - SCOPUS:0028984113
VL - 42
SP - 653
EP - 657
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -