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Flow-injection sandwich ELISA for bioprocess monitoring

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Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalJournal of Automated Methods and Management in Chemistry
Volume21
Issue number4
Publication statusPublished - 1999

Abstract

A fully automated flow-injection immunoassay based on sandwich enzyme-linked immunosorbant assay (ELISA) is described for the model system: protein G-sepharose, rabbit IgG and horseradish peroxidase (HRP)-labelled protein A. After injecting rabbit IgG and HRP-labelled protein A into a cartridge containing protein G-sepharose sequentially, a mixture of hydrogen peroxide and the redox indicator, 2.2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) is passed through the cartridge. The HRP-labelled protein A bound in the cartridge is directly proportional to the concentration of rabbit IgG. The colour variation of ABTS caused during the reaction between HRP and H2O2 in the cartridge is defected photometrically. The whole assay procedure is controlled and evaluated by a computer. Rabbit IgG and HRP-labelled protein A are also detected by a fluorometer, which is introduced into the flow system. In the flow-injection sandwich ELISA, the slope of the calibration curve is 0.4491 in the range of 0 and 300 μg ml-1 rabbit IgG, while it is 0.1274 in the heterogeneous immunoassay. So the flow-injection sandwich ELISA system is found to be more sensitive than a heterogeneous immunoassay for the monitoring of the model protein.

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Flow-injection sandwich ELISA for bioprocess monitoring. / Rhee, Jong I.I.; Hagedorn, Jörg; Scheper, Thomas et al.
In: Journal of Automated Methods and Management in Chemistry, Vol. 21, No. 4, 1999, p. 121-125.

Research output: Contribution to journalArticleResearchpeer review

Rhee JII, Hagedorn J, Scheper T, Schügerl K. Flow-injection sandwich ELISA for bioprocess monitoring. Journal of Automated Methods and Management in Chemistry. 1999;21(4):121-125. doi: 10.1155/S1463924699000140
Rhee, Jong I.I. ; Hagedorn, Jörg ; Scheper, Thomas et al. / Flow-injection sandwich ELISA for bioprocess monitoring. In: Journal of Automated Methods and Management in Chemistry. 1999 ; Vol. 21, No. 4. pp. 121-125.
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AU - Hagedorn, Jörg

AU - Scheper, Thomas

AU - Schügerl, Karl

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