Details
| Original language | English |
|---|---|
| Article number | 18 |
| Journal | AMB EXPRESS |
| Volume | 8 |
| Early online date | 10 Feb 2018 |
| Publication status | E-pub ahead of print - 10 Feb 2018 |
Abstract
Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.
Keywords
- Bioactive peptides, Continuous process, Food protein, Immobilization, Proteases
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: AMB EXPRESS, Vol. 8, 18, 10.02.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Flow rate dependent continuous hydrolysis of protein isolates
AU - Sewczyk, Tim
AU - Hoog Antink, Marieke
AU - Maas, Michael
AU - Kroll, Stephen
AU - Beutel, Sascha
N1 - Funding information: This research project was supported with a funding from the German Research Foundation (Deutsche Forschungsgemeinschaft) with the Grant Number 278836263. We would like to thank Novozymes A/S, Denmark for providing the Alcalase® 2.5 and the German Research Foundation for funding this project.
PY - 2018/2/10
Y1 - 2018/2/10
N2 - Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.
AB - Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.
KW - Bioactive peptides
KW - Continuous process
KW - Food protein
KW - Immobilization
KW - Proteases
UR - http://www.scopus.com/inward/record.url?scp=85041922676&partnerID=8YFLogxK
U2 - 10.1186/s13568-018-0548-9
DO - 10.1186/s13568-018-0548-9
M3 - Article
AN - SCOPUS:85041922676
VL - 8
JO - AMB EXPRESS
JF - AMB EXPRESS
SN - 2191-0855
M1 - 18
ER -