Details
| Original language | English |
|---|---|
| Pages (from-to) | 1251-1255 |
| Number of pages | 5 |
| Journal | Biosensors and Bioelectronics |
| Volume | 13 |
| Issue number | 12 |
| Publication status | Published - 4 Dec 1998 |
Abstract
Sugar beet molasses is a natural resource for various products used in daily life, ranging from sucrose to amino acids for pharmaceutical industry. The separation of molasses into these high value components is performed on a large scale by ion exchange/exclusion chromatography. A biosensor system was set up for the 'in time' analysis of serine and sucrose during molasses desugarisation. D-Serine was analysed with the multi-enzyme system D-serine dehydratase/lactic dehydrogenase and photometric detection of the NADH consumed. Sucrose was determined with invertase/mutarotase/glucose oxidase and the oxygen consumed was monitored amperometrically. An analysis could be performed within 2-5 min by directly injecting samples from the chromatographic process into the flow injection analysis system. The determination range for the sucrose analysis was 0-2.5 gl-1 and for the analysis of D-serine 0-0.5 gl-1. The standard deviation for the measurement of D-serine was 1.7%.
Keywords
- Biosensor, Flow injection analysis, Serine, Sugar beet molasses
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 13, No. 12, 04.12.1998, p. 1251-1255.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Flow injection analysis system for the supervision of industrial chromatographic downstream processing in biotechnology
AU - Sosnitza, P.
AU - Irtel, F.
AU - Ulber, R.
AU - Busse, M.
AU - Faurie, R.
AU - Fischer, L.
AU - Scheper, T.
N1 - Funding information: The research was performed on basis of financial support from the Deutsche Bundestiftung Umwelt (Osnabrück, Germany).
PY - 1998/12/4
Y1 - 1998/12/4
N2 - Sugar beet molasses is a natural resource for various products used in daily life, ranging from sucrose to amino acids for pharmaceutical industry. The separation of molasses into these high value components is performed on a large scale by ion exchange/exclusion chromatography. A biosensor system was set up for the 'in time' analysis of serine and sucrose during molasses desugarisation. D-Serine was analysed with the multi-enzyme system D-serine dehydratase/lactic dehydrogenase and photometric detection of the NADH consumed. Sucrose was determined with invertase/mutarotase/glucose oxidase and the oxygen consumed was monitored amperometrically. An analysis could be performed within 2-5 min by directly injecting samples from the chromatographic process into the flow injection analysis system. The determination range for the sucrose analysis was 0-2.5 gl-1 and for the analysis of D-serine 0-0.5 gl-1. The standard deviation for the measurement of D-serine was 1.7%.
AB - Sugar beet molasses is a natural resource for various products used in daily life, ranging from sucrose to amino acids for pharmaceutical industry. The separation of molasses into these high value components is performed on a large scale by ion exchange/exclusion chromatography. A biosensor system was set up for the 'in time' analysis of serine and sucrose during molasses desugarisation. D-Serine was analysed with the multi-enzyme system D-serine dehydratase/lactic dehydrogenase and photometric detection of the NADH consumed. Sucrose was determined with invertase/mutarotase/glucose oxidase and the oxygen consumed was monitored amperometrically. An analysis could be performed within 2-5 min by directly injecting samples from the chromatographic process into the flow injection analysis system. The determination range for the sucrose analysis was 0-2.5 gl-1 and for the analysis of D-serine 0-0.5 gl-1. The standard deviation for the measurement of D-serine was 1.7%.
KW - Biosensor
KW - Flow injection analysis
KW - Serine
KW - Sugar beet molasses
UR - http://www.scopus.com/inward/record.url?scp=0032436347&partnerID=8YFLogxK
U2 - 10.1016/S0956-5663(98)00084-0
DO - 10.1016/S0956-5663(98)00084-0
M3 - Article
C2 - 9883558
AN - SCOPUS:0032436347
VL - 13
SP - 1251
EP - 1255
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 12
ER -