Details
| Original language | English |
|---|---|
| Pages (from-to) | 399-405 |
| Number of pages | 7 |
| Journal | Enzyme and microbial technology |
| Volume | 9 |
| Issue number | 7 |
| Publication status | Published - Jul 1987 |
Abstract
Double-beam laser flow cytometry has been used to analyse samples from different Saccharomyces cerevisiae cultivation processes (batch and continuous culture). Using this technique, the biological parameters of cell size, and protein, RNA and DNA content can be determined. It is possible to study the budding behaviour from the cell size distribution as well as from DNA analysis. In oscillating continuous yeast cultures, flow cytometry studies were performed in addition to on-line NADH measurements. The effect of cell recycling on yeast metabolism was also the object of cytometric studies. Structured biological data can be obtained by this analytical technique, and the effect of different cultivation conditions on cell biology can be determined.
Keywords
- cell recycle, Flow cytometry, Saccharomyces cerevisiae
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Enzyme and microbial technology, Vol. 9, No. 7, 07.1987, p. 399-405.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Flow cytometric studies during culture of Saccharomyces cerevisiae
AU - Scheper, Th
AU - Hoffmann, H.
AU - Schügerl, K.
PY - 1987/7
Y1 - 1987/7
N2 - Double-beam laser flow cytometry has been used to analyse samples from different Saccharomyces cerevisiae cultivation processes (batch and continuous culture). Using this technique, the biological parameters of cell size, and protein, RNA and DNA content can be determined. It is possible to study the budding behaviour from the cell size distribution as well as from DNA analysis. In oscillating continuous yeast cultures, flow cytometry studies were performed in addition to on-line NADH measurements. The effect of cell recycling on yeast metabolism was also the object of cytometric studies. Structured biological data can be obtained by this analytical technique, and the effect of different cultivation conditions on cell biology can be determined.
AB - Double-beam laser flow cytometry has been used to analyse samples from different Saccharomyces cerevisiae cultivation processes (batch and continuous culture). Using this technique, the biological parameters of cell size, and protein, RNA and DNA content can be determined. It is possible to study the budding behaviour from the cell size distribution as well as from DNA analysis. In oscillating continuous yeast cultures, flow cytometry studies were performed in addition to on-line NADH measurements. The effect of cell recycling on yeast metabolism was also the object of cytometric studies. Structured biological data can be obtained by this analytical technique, and the effect of different cultivation conditions on cell biology can be determined.
KW - cell recycle
KW - Flow cytometry
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0023381802&partnerID=8YFLogxK
U2 - 10.1016/0141-0229(87)90134-7
DO - 10.1016/0141-0229(87)90134-7
M3 - Article
AN - SCOPUS:0023381802
VL - 9
SP - 399
EP - 405
JO - Enzyme and microbial technology
JF - Enzyme and microbial technology
SN - 0141-0229
IS - 7
ER -