Details
| Original language | English |
|---|---|
| Pages (from-to) | 400-404 |
| Number of pages | 5 |
| Journal | Plant cell reports |
| Volume | 17 |
| Issue number | 5 |
| Publication status | Published - Mar 1998 |
Abstract
Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype ('Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content of the diploid plant in the greenhouse was 1.12 pg DNA/2C as estimated in relation to the internal standards tomato nuclei and chicken erythrocytes. In both callus lines the majority of cells contained the same amount of DNA as the initial plant, indicating that no polyploidization has taken place after 5 years of culture on medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 mg/l 6-(γ-γ-dimethylallylamino)purine(zip). Thus, our data suggest that in Cyclamen callus lines there was no strict correlation between the ploidy level and the ability to produce somatic embryos. Furthermore, following the proportion of cells in the three phases of the cell cycle (G0/GI, S, G2/M) during one subculture period of 4 weeks revealed high division activity within the first 2 weeks for both callus lines cultured on the 2,4-D-containing medium. However, when transferred to hormone-free medium, the division activity of the embryogenic cell line decreased markedly, corresponding to the differentiation of somatic embryos. In contrast, for the non-embryogenic callus an increase in cells in the G2/M phase was observed.
Keywords
- Cell cycle, Cyclamen persicum, Flow cytometry, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant cell reports, Vol. 17, No. 5, 03.1998, p. 400-404.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum mill.
T2 - Relation between ploidy level and competence for somatic embryogenesis
AU - Winkelmann, T.
AU - Sangwan, R. S.
AU - Schwenkel, H. G.
PY - 1998/3
Y1 - 1998/3
N2 - Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype ('Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content of the diploid plant in the greenhouse was 1.12 pg DNA/2C as estimated in relation to the internal standards tomato nuclei and chicken erythrocytes. In both callus lines the majority of cells contained the same amount of DNA as the initial plant, indicating that no polyploidization has taken place after 5 years of culture on medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 mg/l 6-(γ-γ-dimethylallylamino)purine(zip). Thus, our data suggest that in Cyclamen callus lines there was no strict correlation between the ploidy level and the ability to produce somatic embryos. Furthermore, following the proportion of cells in the three phases of the cell cycle (G0/GI, S, G2/M) during one subculture period of 4 weeks revealed high division activity within the first 2 weeks for both callus lines cultured on the 2,4-D-containing medium. However, when transferred to hormone-free medium, the division activity of the embryogenic cell line decreased markedly, corresponding to the differentiation of somatic embryos. In contrast, for the non-embryogenic callus an increase in cells in the G2/M phase was observed.
AB - Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype ('Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content of the diploid plant in the greenhouse was 1.12 pg DNA/2C as estimated in relation to the internal standards tomato nuclei and chicken erythrocytes. In both callus lines the majority of cells contained the same amount of DNA as the initial plant, indicating that no polyploidization has taken place after 5 years of culture on medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 mg/l 6-(γ-γ-dimethylallylamino)purine(zip). Thus, our data suggest that in Cyclamen callus lines there was no strict correlation between the ploidy level and the ability to produce somatic embryos. Furthermore, following the proportion of cells in the three phases of the cell cycle (G0/GI, S, G2/M) during one subculture period of 4 weeks revealed high division activity within the first 2 weeks for both callus lines cultured on the 2,4-D-containing medium. However, when transferred to hormone-free medium, the division activity of the embryogenic cell line decreased markedly, corresponding to the differentiation of somatic embryos. In contrast, for the non-embryogenic callus an increase in cells in the G2/M phase was observed.
KW - Cell cycle
KW - Cyclamen persicum
KW - Flow cytometry
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=0031898360&partnerID=8YFLogxK
U2 - 10.1007/s002990050414
DO - 10.1007/s002990050414
M3 - Article
AN - SCOPUS:0031898360
VL - 17
SP - 400
EP - 404
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 5
ER -