Details
| Original language | English |
|---|---|
| Pages (from-to) | 2411-2415 |
| Number of pages | 5 |
| Journal | Archives of virology |
| Volume | 167 |
| Issue number | 11 |
| Early online date | 13 Aug 2022 |
| Publication status | Published - Nov 2022 |
Abstract
The fungus Ilyonectria pseudodestructans belongs to the family Nectriaceae and was found to be part of the endophytic microbiome of apple trees (Malus x domestica, Borkh.) with apple replant disease (ARD). After dsRNA extraction, a mycoviral infection became evident. Here, we report the identification of a new virus, tentatively named “Ilyonectria pseudodestructans chrysovirus 1” (IpCV1), as the first member of the proposed new species “Alphachrysovirus ilyonectriae” within the genus Alphachrysovirus. This is the first report of a chrysovirus infecting a member of the fungal genus Ilyonectria. IpCV1 has a tripartite dsRNA genome with a total length of 8944 bp. The segments are 3439 bp, 2850 bp, and 2655 bp in length, and each dsRNA carries a single ORF. The encoded viral proteins are a 125.92-kDa RNA-dependent RNA polymerase, a 100.75-kDa coat protein, and one protein of unknown function with a predicted molecular mass of 93.04 kDa. The 5´ and 3´ UTRs are comparatively short and are 79 to 91 bp and 62 to 148 bp in length, respectively. This study provides the basis for further investigations of the impact of IpCV1 on its host and the etiology of ARD.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Archives of virology, Vol. 167, No. 11, 11.2022, p. 2411-2415.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - First report of a chrysovirus infecting a member of the fungal genus Ilyonectria
AU - Pielhop, Tom P.
AU - Popp, Carolin
AU - Knierim, Dennis
AU - Margaria, Paolo
AU - Maiß, Edgar
N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. This work was funded by the Federal Ministry of Education and Research and is part of the ORDIAmur network (FKZ: 031B0025A), which is allocated to the BONARES program.
PY - 2022/11
Y1 - 2022/11
N2 - The fungus Ilyonectria pseudodestructans belongs to the family Nectriaceae and was found to be part of the endophytic microbiome of apple trees (Malus x domestica, Borkh.) with apple replant disease (ARD). After dsRNA extraction, a mycoviral infection became evident. Here, we report the identification of a new virus, tentatively named “Ilyonectria pseudodestructans chrysovirus 1” (IpCV1), as the first member of the proposed new species “Alphachrysovirus ilyonectriae” within the genus Alphachrysovirus. This is the first report of a chrysovirus infecting a member of the fungal genus Ilyonectria. IpCV1 has a tripartite dsRNA genome with a total length of 8944 bp. The segments are 3439 bp, 2850 bp, and 2655 bp in length, and each dsRNA carries a single ORF. The encoded viral proteins are a 125.92-kDa RNA-dependent RNA polymerase, a 100.75-kDa coat protein, and one protein of unknown function with a predicted molecular mass of 93.04 kDa. The 5´ and 3´ UTRs are comparatively short and are 79 to 91 bp and 62 to 148 bp in length, respectively. This study provides the basis for further investigations of the impact of IpCV1 on its host and the etiology of ARD.
AB - The fungus Ilyonectria pseudodestructans belongs to the family Nectriaceae and was found to be part of the endophytic microbiome of apple trees (Malus x domestica, Borkh.) with apple replant disease (ARD). After dsRNA extraction, a mycoviral infection became evident. Here, we report the identification of a new virus, tentatively named “Ilyonectria pseudodestructans chrysovirus 1” (IpCV1), as the first member of the proposed new species “Alphachrysovirus ilyonectriae” within the genus Alphachrysovirus. This is the first report of a chrysovirus infecting a member of the fungal genus Ilyonectria. IpCV1 has a tripartite dsRNA genome with a total length of 8944 bp. The segments are 3439 bp, 2850 bp, and 2655 bp in length, and each dsRNA carries a single ORF. The encoded viral proteins are a 125.92-kDa RNA-dependent RNA polymerase, a 100.75-kDa coat protein, and one protein of unknown function with a predicted molecular mass of 93.04 kDa. The 5´ and 3´ UTRs are comparatively short and are 79 to 91 bp and 62 to 148 bp in length, respectively. This study provides the basis for further investigations of the impact of IpCV1 on its host and the etiology of ARD.
UR - http://www.scopus.com/inward/record.url?scp=85135800778&partnerID=8YFLogxK
U2 - 10.1007/s00705-022-05551-2
DO - 10.1007/s00705-022-05551-2
M3 - Article
C2 - 35963918
AN - SCOPUS:85135800778
VL - 167
SP - 2411
EP - 2415
JO - Archives of virology
JF - Archives of virology
SN - 0304-8608
IS - 11
ER -