Details
| Original language | English |
|---|---|
| Pages (from-to) | 1508-1512 |
| Number of pages | 5 |
| Journal | CHEMBIOCHEM |
| Volume | 11 |
| Issue number | 11 |
| Publication status | Published - 26 Jul 2010 |
| Externally published | Yes |
Abstract
(Chemical Equation Presented) Fungal chemical factory: Controlled expression of the complete tenellin biosynthetic pathway from Beauveria bassiana was achieved in the heterologous host Aspergillus oryzae by replacing the promoters of all four of its component genes with the A. oryzae amyB promoter; this produces tenellin at over five times the concentration of the native host.
Keywords
- Biosynthesis, Cytochrome P450, Gene expression, Natural products, Polyketides
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Organic Chemistry
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In: CHEMBIOCHEM, Vol. 11, No. 11, 26.07.2010, p. 1508-1512.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - First heterologous reconstruction of a complete functional fungal biosynthetic multigene cluster
AU - Heneghan, Mary N.
AU - Yakasai, Ahmed A.
AU - Halo, Laura M.
AU - Song, Zhongshu
AU - Bailey, Andrew M.
AU - Simpson, Thomas J.
AU - Cox, Russell J.
AU - Lazarus, Colin M.
PY - 2010/7/26
Y1 - 2010/7/26
N2 - (Chemical Equation Presented) Fungal chemical factory: Controlled expression of the complete tenellin biosynthetic pathway from Beauveria bassiana was achieved in the heterologous host Aspergillus oryzae by replacing the promoters of all four of its component genes with the A. oryzae amyB promoter; this produces tenellin at over five times the concentration of the native host.
AB - (Chemical Equation Presented) Fungal chemical factory: Controlled expression of the complete tenellin biosynthetic pathway from Beauveria bassiana was achieved in the heterologous host Aspergillus oryzae by replacing the promoters of all four of its component genes with the A. oryzae amyB promoter; this produces tenellin at over five times the concentration of the native host.
KW - Biosynthesis
KW - Cytochrome P450
KW - Gene expression
KW - Natural products
KW - Polyketides
UR - http://www.scopus.com/inward/record.url?scp=77955148530&partnerID=8YFLogxK
U2 - 10.1002/cbic.201000259
DO - 10.1002/cbic.201000259
M3 - Article
C2 - 20575135
AN - SCOPUS:77955148530
VL - 11
SP - 1508
EP - 1512
JO - CHEMBIOCHEM
JF - CHEMBIOCHEM
SN - 1439-4227
IS - 11
ER -