First genome edited poinsettias: targeted mutagenesis of flavonoid 3′-hydroxylase using CRISPR/Cas9 results in a colour shift

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Daria Nitarska
  • Robert Boehm
  • Thomas Debener
  • Rares Calin Lucaciu
  • Heidi Halbwirth

Research Organisations

External Research Organisations

  • TU Wien (TUW)
  • Klemm & Sohn GmbH co. KG
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Details

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalPlant Cell, Tissue and Organ Culture
Volume147
Issue number1
Early online date26 May 2021
Publication statusPublished - Oct 2021

Abstract

The CRISPR/Cas9 system is a remarkably promising tool for targeted gene mutagenesis, and becoming ever more popular for modification of ornamental plants. In this study we performed the knockout of flavonoid 3′-hydroxylase (F3′H) with application of CRISPR/Cas9 in the red flowering poinsettia (Euphorbia pulcherrima) cultivar ‘Christmas Eve’, in order to obtain plants with orange bract colour, which accumulate prevalently pelargonidin. F3′H is an enzyme that is necessary for formation of cyanidin type anthocyanins, which are responsible for the red colour of poinsettia bracts. Even though F3′H was not completely inactivated, the bract colour of transgenic plants changed from vivid red (RHS 45B) to vivid reddish orange (RHS 33A), and cyanidin levels decreased significantly compared with the wild type. In the genetically modified plants, an increased ratio of pelargonidin to cyanidin was observed. By cloning and expression of mutated proteins, the lack of F3′H activity was confirmed. This confirms that a loss of function mutation in the poinsettia F3′H gene is sufficient for obtaining poinsettia with orange bract colour. This is the first report of successful use of CRISPR/Cas9 for genome editing in poinsettia.

Keywords

    Breeding, Euphorbia pulcherrima, Orange bract colour, Pelargonidin

ASJC Scopus subject areas

Cite this

First genome edited poinsettias: targeted mutagenesis of flavonoid 3′-hydroxylase using CRISPR/Cas9 results in a colour shift. / Nitarska, Daria; Boehm, Robert; Debener, Thomas et al.
In: Plant Cell, Tissue and Organ Culture, Vol. 147, No. 1, 10.2021, p. 49-60.

Research output: Contribution to journalArticleResearchpeer review

Nitarska D, Boehm R, Debener T, Lucaciu RC, Halbwirth H. First genome edited poinsettias: targeted mutagenesis of flavonoid 3′-hydroxylase using CRISPR/Cas9 results in a colour shift. Plant Cell, Tissue and Organ Culture. 2021 Oct;147(1):49-60. Epub 2021 May 26. doi: 10.1007/s11240-021-02103-5
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title = "First genome edited poinsettias: targeted mutagenesis of flavonoid 3′-hydroxylase using CRISPR/Cas9 results in a colour shift",
abstract = "The CRISPR/Cas9 system is a remarkably promising tool for targeted gene mutagenesis, and becoming ever more popular for modification of ornamental plants. In this study we performed the knockout of flavonoid 3′-hydroxylase (F3′H) with application of CRISPR/Cas9 in the red flowering poinsettia (Euphorbia pulcherrima) cultivar {\textquoteleft}Christmas Eve{\textquoteright}, in order to obtain plants with orange bract colour, which accumulate prevalently pelargonidin. F3′H is an enzyme that is necessary for formation of cyanidin type anthocyanins, which are responsible for the red colour of poinsettia bracts. Even though F3′H was not completely inactivated, the bract colour of transgenic plants changed from vivid red (RHS 45B) to vivid reddish orange (RHS 33A), and cyanidin levels decreased significantly compared with the wild type. In the genetically modified plants, an increased ratio of pelargonidin to cyanidin was observed. By cloning and expression of mutated proteins, the lack of F3′H activity was confirmed. This confirms that a loss of function mutation in the poinsettia F3′H gene is sufficient for obtaining poinsettia with orange bract colour. This is the first report of successful use of CRISPR/Cas9 for genome editing in poinsettia.",
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