Details
| Original language | English |
|---|---|
| Pages (from-to) | 737-742 |
| Number of pages | 6 |
| Journal | Pest management science |
| Volume | 57 |
| Issue number | 8 |
| Early online date | 18 Jul 2001 |
| Publication status | Published - 2001 |
| Externally published | Yes |
Abstract
Effects of temperature, humidity, rewetting and removal of deposits on penetration of NAA [2-(1-naphthyl) acetic acid] through isolated tomato (Lycopersicon esculentum Mill) fruit cuticles were studied using a finite dose diffusion system. In this system, an aqueous 5-μl droplet (0.1 mM NAA in 20 mM citric acid buffer) is applied to the outer surface of a cuticle, which is mounted in a glass diffusion half-cell. The cell wall surface is in contact with a receiver solution (20mM citrate). Penetration is monitored by repeated sampling of the receiver solution. Droplets appeared dry on visual inspection within 1 h of application, but significant NAA penetration continued after droplet drying. Maximum rates of NAA penetration increased exponentially as temperature was increased (from 5° to 35°C), the energy of activation averaging 153 (±11.6) kJmol-1. At 35°C, penetration reached a plateau within 10h of application (at 91.1 (±1.0)% of dose applied) while at 5°C penetration after 800h reached only 30.2 (±7.5)%. Increasing relative humidity from 20 to 80% increased maximum rates [from 1.0 (±0.21) to 2.7(±0.80)% h-1] and penetration at 120h after application [from 36.8(±2.1) to 64.3(±3.7)%]. Rewetting deposits at 120, 240 and 360h after application resulted in increased NAA penetration. However, amounts and rates of NAA penetration progressively decreased with each subsequent rewetting. Removal of deposits by cellulose acetate stripping at various times after droplet application resulted in a rapid decrease in NAA penetration. NAA penetration following deposit removal was always less than 6.1% of the amount of NAA applied and averaged 0.5 (±0.2)% when deposits were removed immediately after droplet drying.
Keywords
- Absorption, Deposit rewetting, Droplet, Foliar uptake, Humidity, Naphthylacetic acid, Temperature
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Insect Science
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In: Pest management science, Vol. 57, No. 8, 2001, p. 737-742.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Finite dose diffusion studies
T2 - III. Effects of temperature, humidity and deposit manipulation on NAA penetration through isolated tomato fruit cuticles
AU - Knoche, Moritz
AU - Bukovac, Martin J.
PY - 2001
Y1 - 2001
N2 - Effects of temperature, humidity, rewetting and removal of deposits on penetration of NAA [2-(1-naphthyl) acetic acid] through isolated tomato (Lycopersicon esculentum Mill) fruit cuticles were studied using a finite dose diffusion system. In this system, an aqueous 5-μl droplet (0.1 mM NAA in 20 mM citric acid buffer) is applied to the outer surface of a cuticle, which is mounted in a glass diffusion half-cell. The cell wall surface is in contact with a receiver solution (20mM citrate). Penetration is monitored by repeated sampling of the receiver solution. Droplets appeared dry on visual inspection within 1 h of application, but significant NAA penetration continued after droplet drying. Maximum rates of NAA penetration increased exponentially as temperature was increased (from 5° to 35°C), the energy of activation averaging 153 (±11.6) kJmol-1. At 35°C, penetration reached a plateau within 10h of application (at 91.1 (±1.0)% of dose applied) while at 5°C penetration after 800h reached only 30.2 (±7.5)%. Increasing relative humidity from 20 to 80% increased maximum rates [from 1.0 (±0.21) to 2.7(±0.80)% h-1] and penetration at 120h after application [from 36.8(±2.1) to 64.3(±3.7)%]. Rewetting deposits at 120, 240 and 360h after application resulted in increased NAA penetration. However, amounts and rates of NAA penetration progressively decreased with each subsequent rewetting. Removal of deposits by cellulose acetate stripping at various times after droplet application resulted in a rapid decrease in NAA penetration. NAA penetration following deposit removal was always less than 6.1% of the amount of NAA applied and averaged 0.5 (±0.2)% when deposits were removed immediately after droplet drying.
AB - Effects of temperature, humidity, rewetting and removal of deposits on penetration of NAA [2-(1-naphthyl) acetic acid] through isolated tomato (Lycopersicon esculentum Mill) fruit cuticles were studied using a finite dose diffusion system. In this system, an aqueous 5-μl droplet (0.1 mM NAA in 20 mM citric acid buffer) is applied to the outer surface of a cuticle, which is mounted in a glass diffusion half-cell. The cell wall surface is in contact with a receiver solution (20mM citrate). Penetration is monitored by repeated sampling of the receiver solution. Droplets appeared dry on visual inspection within 1 h of application, but significant NAA penetration continued after droplet drying. Maximum rates of NAA penetration increased exponentially as temperature was increased (from 5° to 35°C), the energy of activation averaging 153 (±11.6) kJmol-1. At 35°C, penetration reached a plateau within 10h of application (at 91.1 (±1.0)% of dose applied) while at 5°C penetration after 800h reached only 30.2 (±7.5)%. Increasing relative humidity from 20 to 80% increased maximum rates [from 1.0 (±0.21) to 2.7(±0.80)% h-1] and penetration at 120h after application [from 36.8(±2.1) to 64.3(±3.7)%]. Rewetting deposits at 120, 240 and 360h after application resulted in increased NAA penetration. However, amounts and rates of NAA penetration progressively decreased with each subsequent rewetting. Removal of deposits by cellulose acetate stripping at various times after droplet application resulted in a rapid decrease in NAA penetration. NAA penetration following deposit removal was always less than 6.1% of the amount of NAA applied and averaged 0.5 (±0.2)% when deposits were removed immediately after droplet drying.
KW - Absorption
KW - Deposit rewetting
KW - Droplet
KW - Foliar uptake
KW - Humidity
KW - Naphthylacetic acid
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=0034899283&partnerID=8YFLogxK
U2 - 10.1002/ps.351
DO - 10.1002/ps.351
M3 - Article
C2 - 11517728
AN - SCOPUS:0034899283
VL - 57
SP - 737
EP - 742
JO - Pest management science
JF - Pest management science
SN - 1526-498X
IS - 8
ER -