Details
| Original language | English |
|---|---|
| Pages (from-to) | 38-46 |
| Number of pages | 9 |
| Journal | Bioresource technology |
| Volume | 220 |
| Publication status | Published - 12 Aug 2016 |
Abstract
Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.
Keywords
- Agro-industrial side-streams, Basidiomycota, Benzoic acid ester, Feruloyl esterase, Pichia pastoris
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Environmental Science(all)
- Environmental Engineering
- Energy(all)
- Renewable Energy, Sustainability and the Environment
- Environmental Science(all)
- Waste Management and Disposal
Sustainable Development Goals
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In: Bioresource technology, Vol. 220, 12.08.2016, p. 38-46.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Feruloyl esterases from Schizophyllum commune to treat food industry side-streams
AU - Nieter, Annabel
AU - Kelle, Sebastian
AU - Linke, Diana
AU - Berger, Ralf G.
N1 - Funding information: Support of the work by the Federal Ministry of Education and Research cluster Biokatalyse2021 (P 37) and by M. Bunzel (Department of Food Chemistry and Phytochemistry, KIT, Karlsruhe, Germany) is gratefully acknowledged.
PY - 2016/8/12
Y1 - 2016/8/12
N2 - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.
AB - Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000 U L−1. The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.
KW - Agro-industrial side-streams
KW - Basidiomycota
KW - Benzoic acid ester
KW - Feruloyl esterase
KW - Pichia pastoris
UR - http://www.scopus.com/inward/record.url?scp=84989907860&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.08.045
DO - 10.1016/j.biortech.2016.08.045
M3 - Article
C2 - 27566510
AN - SCOPUS:84989907860
VL - 220
SP - 38
EP - 46
JO - Bioresource technology
JF - Bioresource technology
SN - 0960-8524
ER -