Details
| Original language | English |
|---|---|
| Title of host publication | Photonic Therapeutics and Diagnostics VI |
| Publication status | Published - 2 Mar 2010 |
| Externally published | Yes |
| Event | Photonic Therapeutics and Diagnostics VI - San Francisco, CA, United States Duration: 23 Jan 2010 → 25 Jan 2010 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7548 |
| ISSN (Print) | 1605-7422 |
Abstract
Introduction: Titanium implants can be regarded as the current gold standard for restoration of sound transmission in the middle ear following destruction of the ossicular chain by chronic inflammation. Many efforts have been made to improve prosthesis design, while less attention had been given to the role of the interface. We present a study on chemical nanocoating on microstructured titanium contact surface with bioactive protein. Materials and Methods: Titanium samples of 5mm diameter and 0,25mm thickness were structured by means of a Ti:Sapphire femtosecond laser operating at 970nm with parallel lines of 5μm depth, 5μm width and 10μm inter-groove distance. In addition, various nanolayers were applied to titanium samples by aminosilanization, to which Star-Polyethylene glycole (Star-PEG) molecules plus biomarkers (e.g. RGD peptide sequence) were linked. Results: Chondrocytes could be cultured on microstructured surfaces without reduced rate of vital / dead cells compared to native surfaces. Chondrocytes also showed contact guidance by growing along ridges particularly on 5μm lines. On nanocoated titanium samples, first results showed a strong effect of Star-PEG suppressing unspecific protein absorption, while RGD peptide sequence did not promote chondrocyte cell growth. Discussion: According to these results, the idea of promoting cell growth on titanium prosthesis contact surfaces compared to non-contact surfaces (e.g. prosthesis shaft) by nanocoating is practicable. However, relative selectivity induced by microstructures for growth of chondrocytes compared to fibrocytes is subject to further evaluation.
Keywords
- Biosignal, Cell culture, Chondrocyte, Femtosecond laser, Fibrocyte, Microstructure, Microsurgery, Otology
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Photonic Therapeutics and Diagnostics VI. 2010. 75482Y (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7548).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Femtosecond laser microstructuring and bioactive nanocoating of titanium surfaces in relation to chondrocyte growth
AU - Ilgner, Justus
AU - Biedron, Slavomir
AU - Fadeeva, Elena
AU - Chichkov, Boris
AU - Klee, Doris
AU - Loos, Anneke
AU - Sowa-Söhle, Eveline
AU - Westhofen, Martin
PY - 2010/3/2
Y1 - 2010/3/2
N2 - Introduction: Titanium implants can be regarded as the current gold standard for restoration of sound transmission in the middle ear following destruction of the ossicular chain by chronic inflammation. Many efforts have been made to improve prosthesis design, while less attention had been given to the role of the interface. We present a study on chemical nanocoating on microstructured titanium contact surface with bioactive protein. Materials and Methods: Titanium samples of 5mm diameter and 0,25mm thickness were structured by means of a Ti:Sapphire femtosecond laser operating at 970nm with parallel lines of 5μm depth, 5μm width and 10μm inter-groove distance. In addition, various nanolayers were applied to titanium samples by aminosilanization, to which Star-Polyethylene glycole (Star-PEG) molecules plus biomarkers (e.g. RGD peptide sequence) were linked. Results: Chondrocytes could be cultured on microstructured surfaces without reduced rate of vital / dead cells compared to native surfaces. Chondrocytes also showed contact guidance by growing along ridges particularly on 5μm lines. On nanocoated titanium samples, first results showed a strong effect of Star-PEG suppressing unspecific protein absorption, while RGD peptide sequence did not promote chondrocyte cell growth. Discussion: According to these results, the idea of promoting cell growth on titanium prosthesis contact surfaces compared to non-contact surfaces (e.g. prosthesis shaft) by nanocoating is practicable. However, relative selectivity induced by microstructures for growth of chondrocytes compared to fibrocytes is subject to further evaluation.
AB - Introduction: Titanium implants can be regarded as the current gold standard for restoration of sound transmission in the middle ear following destruction of the ossicular chain by chronic inflammation. Many efforts have been made to improve prosthesis design, while less attention had been given to the role of the interface. We present a study on chemical nanocoating on microstructured titanium contact surface with bioactive protein. Materials and Methods: Titanium samples of 5mm diameter and 0,25mm thickness were structured by means of a Ti:Sapphire femtosecond laser operating at 970nm with parallel lines of 5μm depth, 5μm width and 10μm inter-groove distance. In addition, various nanolayers were applied to titanium samples by aminosilanization, to which Star-Polyethylene glycole (Star-PEG) molecules plus biomarkers (e.g. RGD peptide sequence) were linked. Results: Chondrocytes could be cultured on microstructured surfaces without reduced rate of vital / dead cells compared to native surfaces. Chondrocytes also showed contact guidance by growing along ridges particularly on 5μm lines. On nanocoated titanium samples, first results showed a strong effect of Star-PEG suppressing unspecific protein absorption, while RGD peptide sequence did not promote chondrocyte cell growth. Discussion: According to these results, the idea of promoting cell growth on titanium prosthesis contact surfaces compared to non-contact surfaces (e.g. prosthesis shaft) by nanocoating is practicable. However, relative selectivity induced by microstructures for growth of chondrocytes compared to fibrocytes is subject to further evaluation.
KW - Biosignal
KW - Cell culture
KW - Chondrocyte
KW - Femtosecond laser
KW - Fibrocyte
KW - Microstructure
KW - Microsurgery
KW - Otology
UR - http://www.scopus.com/inward/record.url?scp=79751471347&partnerID=8YFLogxK
U2 - 10.1117/12.847361
DO - 10.1117/12.847361
M3 - Conference contribution
AN - SCOPUS:79751471347
SN - 9780819479440
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics VI
T2 - Photonic Therapeutics and Diagnostics VI
Y2 - 23 January 2010 through 25 January 2010
ER -