Details
| Original language | English |
|---|---|
| Pages (from-to) | 7520-7528 |
| Number of pages | 9 |
| Journal | LANGMUIR |
| Volume | 35 |
| Issue number | 23 |
| Early online date | 2 Dec 2018 |
| Publication status | Published - 11 Jun 2019 |
Abstract
Cellular membranes are exposed to extreme conditions during the processing steps involved in cryopreservation (and freeze-drying) of cells. The first processing step involves adding protective agents. Exposing cells to protective agents causes fluxes of both water and solutes (i.e., permeating cryoprotective agents) across the cellular membrane, resulting in cell volume changes and possibly osmotic stress. In addition, protective molecules may interact with lipids, which may lead to membrane structural changes and permeabilization. After loading with protective agents, subsequent freezing exposes cells to severe osmotic and mechanical stresses, caused by extra and/or intracellular ice formation and a drastically increased solute concentration in the unfrozen fraction. Furthermore, cellular membranes undergo thermotropic and lyotropic phase transitions during cooling and freezing, which drastically alter the membrane permeability and its barrier function. In this article, it is shown that membrane permeability to water and solutes is dependent on the temperature, medium osmolality, types of solutes present, cell hydration level, and absence or presence of ice. Freezing most drastically alters the membrane permeability barrier function, which is reflected as a change in the activation energy for water transport. In addition, membranes become temporarily leaky during freezing-induced fluid-to-gel membrane phase transitions, resulting in the uptake of impermeable solutes.
ASJC Scopus subject areas
- Materials Science(all)
- Physics and Astronomy(all)
- Condensed Matter Physics
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Chemistry(all)
- Spectroscopy
- Chemistry(all)
- Electrochemistry
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In: LANGMUIR, Vol. 35, No. 23, 11.06.2019, p. 7520-7528.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Factors Affecting the Membrane Permeability Barrier Function of Cells during Preservation Technologies
AU - Wolkers, Willem F.
AU - Oldenhof, Harriëtte
AU - Tang, Fengrui
AU - Han, Jiale
AU - Bigalk, Judith
AU - Sieme, Harald
N1 - Funding Information: This work was financially supported by the German Research Foundation (DFG: Deutsche Forschungsgemeinschaft) via the Cluster of Excellence ‘”From regenerative biology to reconstructive therapy” (REBIRTH) and grants WO1735/6-1 and SI1462/4-1. Bulat Sydykov and Arielle Keller Brunatto are acknowledged for their help with fitting data and mathematical modeling with MATLAB. All previous and current members of the laboratories of W.F.W. and H.S. are acknowledged for their contributions.
PY - 2019/6/11
Y1 - 2019/6/11
N2 - Cellular membranes are exposed to extreme conditions during the processing steps involved in cryopreservation (and freeze-drying) of cells. The first processing step involves adding protective agents. Exposing cells to protective agents causes fluxes of both water and solutes (i.e., permeating cryoprotective agents) across the cellular membrane, resulting in cell volume changes and possibly osmotic stress. In addition, protective molecules may interact with lipids, which may lead to membrane structural changes and permeabilization. After loading with protective agents, subsequent freezing exposes cells to severe osmotic and mechanical stresses, caused by extra and/or intracellular ice formation and a drastically increased solute concentration in the unfrozen fraction. Furthermore, cellular membranes undergo thermotropic and lyotropic phase transitions during cooling and freezing, which drastically alter the membrane permeability and its barrier function. In this article, it is shown that membrane permeability to water and solutes is dependent on the temperature, medium osmolality, types of solutes present, cell hydration level, and absence or presence of ice. Freezing most drastically alters the membrane permeability barrier function, which is reflected as a change in the activation energy for water transport. In addition, membranes become temporarily leaky during freezing-induced fluid-to-gel membrane phase transitions, resulting in the uptake of impermeable solutes.
AB - Cellular membranes are exposed to extreme conditions during the processing steps involved in cryopreservation (and freeze-drying) of cells. The first processing step involves adding protective agents. Exposing cells to protective agents causes fluxes of both water and solutes (i.e., permeating cryoprotective agents) across the cellular membrane, resulting in cell volume changes and possibly osmotic stress. In addition, protective molecules may interact with lipids, which may lead to membrane structural changes and permeabilization. After loading with protective agents, subsequent freezing exposes cells to severe osmotic and mechanical stresses, caused by extra and/or intracellular ice formation and a drastically increased solute concentration in the unfrozen fraction. Furthermore, cellular membranes undergo thermotropic and lyotropic phase transitions during cooling and freezing, which drastically alter the membrane permeability and its barrier function. In this article, it is shown that membrane permeability to water and solutes is dependent on the temperature, medium osmolality, types of solutes present, cell hydration level, and absence or presence of ice. Freezing most drastically alters the membrane permeability barrier function, which is reflected as a change in the activation energy for water transport. In addition, membranes become temporarily leaky during freezing-induced fluid-to-gel membrane phase transitions, resulting in the uptake of impermeable solutes.
UR - http://www.scopus.com/inward/record.url?scp=85058647930&partnerID=8YFLogxK
U2 - 10.1021/acs.langmuir.8b02852
DO - 10.1021/acs.langmuir.8b02852
M3 - Article
C2 - 30501184
AN - SCOPUS:85058647930
VL - 35
SP - 7520
EP - 7528
JO - LANGMUIR
JF - LANGMUIR
SN - 0743-7463
IS - 23
ER -