Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

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Authors

External Research Organisations

  • Nanjing University of Technology
  • Northwestern Polytechnical University
  • Technische Universität Dresden
  • University of Southern Denmark
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Original languageEnglish
Article number714
JournalFrontiers in Bioengineering and Biotechnology
Volume8
Publication statusPublished - 7 Jul 2020
Externally publishedYes

Abstract

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

Keywords

    biocatalysis, enzymatic reactions, heterogeneous catalysis, metal–organic frameworks, size-selective catalysis

ASJC Scopus subject areas

Cite this

Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent. / Wang, Yangxin; Zhang, Ningning; Tan, Deming et al.
In: Frontiers in Bioengineering and Biotechnology, Vol. 8, 714, 07.07.2020.

Research output: Contribution to journalArticleResearchpeer review

Wang Y, Zhang N, Tan D, Qi Z, Wu C. Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent. Frontiers in Bioengineering and Biotechnology. 2020 Jul 7;8:714. doi: 10.3389/fbioe.2020.00714
Wang, Yangxin ; Zhang, Ningning ; Tan, Deming et al. / Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent. In: Frontiers in Bioengineering and Biotechnology. 2020 ; Vol. 8.
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title = "Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent",
abstract = "In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.",
keywords = "biocatalysis, enzymatic reactions, heterogeneous catalysis, metal–organic frameworks, size-selective catalysis",
author = "Yangxin Wang and Ningning Zhang and Deming Tan and Zhenhui Qi and Changzhu Wu",
note = "Funding Information: We thank Prof. Marion B. Ansorge-Schumacher (TU Dresden) for valuable discussions and support and Prof. Rainer Jordan (TU Dresden) for providing the lab facility. Funding. We acknowledge financial support from the Deutsche Forschungsgemeinschaft (WU 814/1-1). This work was also financially supported by the Thousand Talents Program of China (1800-16GH030121), Key R&D Program of Shaanxi Province (2019KW-031 and 2019KW-038), China Postdoctoral Science Foundation (2017M623231), and Fundamental Research Funds for the Central Universities (3102018zy051). ",
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AU - Wang, Yangxin

AU - Zhang, Ningning

AU - Tan, Deming

AU - Qi, Zhenhui

AU - Wu, Changzhu

N1 - Funding Information: We thank Prof. Marion B. Ansorge-Schumacher (TU Dresden) for valuable discussions and support and Prof. Rainer Jordan (TU Dresden) for providing the lab facility. Funding. We acknowledge financial support from the Deutsche Forschungsgemeinschaft (WU 814/1-1). This work was also financially supported by the Thousand Talents Program of China (1800-16GH030121), Key R&D Program of Shaanxi Province (2019KW-031 and 2019KW-038), China Postdoctoral Science Foundation (2017M623231), and Fundamental Research Funds for the Central Universities (3102018zy051).

PY - 2020/7/7

Y1 - 2020/7/7

N2 - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

AB - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

KW - biocatalysis

KW - enzymatic reactions

KW - heterogeneous catalysis

KW - metal–organic frameworks

KW - size-selective catalysis

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