Details
| Original language | English |
|---|---|
| Pages (from-to) | 4147-4161 |
| Number of pages | 15 |
| Journal | Biomedical optics express |
| Volume | 15 |
| Issue number | 7 |
| Publication status | Published - 7 Jun 2024 |
Abstract
We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot's (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Biomedical optics express, Vol. 15, No. 7, 07.06.2024, p. 4147-4161.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection
AU - Kishore, Anusha
AU - Varughese, Arun Mathew
AU - Roth, Bernhard
AU - Zeilinger, Carsten
N1 - Publisher Copyright: © 2024 Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License.
PY - 2024/6/7
Y1 - 2024/6/7
N2 - We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot's (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.
AB - We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot's (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.
UR - http://www.scopus.com/inward/record.url?scp=85197432457&partnerID=8YFLogxK
U2 - 10.1364/BOE.527338
DO - 10.1364/BOE.527338
M3 - Article
AN - SCOPUS:85197432457
VL - 15
SP - 4147
EP - 4161
JO - Biomedical optics express
JF - Biomedical optics express
SN - 2156-7085
IS - 7
ER -