Details
| Original language | English |
|---|---|
| Pages (from-to) | 487-497 |
| Number of pages | 11 |
| Journal | MGG Molecular & General Genetics |
| Volume | 249 |
| Issue number | 5 |
| Publication status | Published - 1 Sept 1995 |
| Externally published | Yes |
Abstract
Two new genes, designated exsA and exsB, were identified adjacent to the 24 kb exo gene cluster of Rhizobium meliloti, which is involved in succinoglycan (EPS I) biosynthesis. The derived amino acid sequence of ExsA displayed significant homologies to ATP binding cassette (ABC) transporter proteins. R. meliloti strains mutated in exsA were characterized by a decreased ratio of HMW to LMW EPS I, indicating a function for ExsA in EPS I biosynthesis. The R. meliloti NdvA protein, which is involved in the transport of cyclic β-(1,2)-glucans, was identified as the closest homologue of ExsA. R. meliloti exsB mutants produced a three-fold increased amount of EPS I in comparison to the wild-type strain. In contrast, high copy number of exsB resulted in a decrease in the EPS I level to 20% of wild type, indicating that the exsB gene product can negatively influence EPS I biosynthesis. It was demonstrated that this influence is not due to transcriptional regulation of the exo genes by the exsB gene product. By plasmid integration it was shown that exsA and exsB represent monocistronic transcription units.
Keywords
- ATP binding domain, Regulation, Rhizobium meliloti, Succinoglycan
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: MGG Molecular & General Genetics, Vol. 249, No. 5, 01.09.1995, p. 487-497.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster
T2 - identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis
AU - Becker, Anke
AU - Küster, Helge
AU - Niehaus, Karsten
AU - Pühler, Alfred
PY - 1995/9/1
Y1 - 1995/9/1
N2 - Two new genes, designated exsA and exsB, were identified adjacent to the 24 kb exo gene cluster of Rhizobium meliloti, which is involved in succinoglycan (EPS I) biosynthesis. The derived amino acid sequence of ExsA displayed significant homologies to ATP binding cassette (ABC) transporter proteins. R. meliloti strains mutated in exsA were characterized by a decreased ratio of HMW to LMW EPS I, indicating a function for ExsA in EPS I biosynthesis. The R. meliloti NdvA protein, which is involved in the transport of cyclic β-(1,2)-glucans, was identified as the closest homologue of ExsA. R. meliloti exsB mutants produced a three-fold increased amount of EPS I in comparison to the wild-type strain. In contrast, high copy number of exsB resulted in a decrease in the EPS I level to 20% of wild type, indicating that the exsB gene product can negatively influence EPS I biosynthesis. It was demonstrated that this influence is not due to transcriptional regulation of the exo genes by the exsB gene product. By plasmid integration it was shown that exsA and exsB represent monocistronic transcription units.
AB - Two new genes, designated exsA and exsB, were identified adjacent to the 24 kb exo gene cluster of Rhizobium meliloti, which is involved in succinoglycan (EPS I) biosynthesis. The derived amino acid sequence of ExsA displayed significant homologies to ATP binding cassette (ABC) transporter proteins. R. meliloti strains mutated in exsA were characterized by a decreased ratio of HMW to LMW EPS I, indicating a function for ExsA in EPS I biosynthesis. The R. meliloti NdvA protein, which is involved in the transport of cyclic β-(1,2)-glucans, was identified as the closest homologue of ExsA. R. meliloti exsB mutants produced a three-fold increased amount of EPS I in comparison to the wild-type strain. In contrast, high copy number of exsB resulted in a decrease in the EPS I level to 20% of wild type, indicating that the exsB gene product can negatively influence EPS I biosynthesis. It was demonstrated that this influence is not due to transcriptional regulation of the exo genes by the exsB gene product. By plasmid integration it was shown that exsA and exsB represent monocistronic transcription units.
KW - ATP binding domain
KW - Regulation
KW - Rhizobium meliloti
KW - Succinoglycan
UR - http://www.scopus.com/inward/record.url?scp=0029592713&partnerID=8YFLogxK
U2 - 10.1007/BF00290574
DO - 10.1007/BF00290574
M3 - Article
C2 - 8544814
AN - SCOPUS:0029592713
VL - 249
SP - 487
EP - 497
JO - MGG Molecular & General Genetics
JF - MGG Molecular & General Genetics
SN - 0026-8925
IS - 5
ER -