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Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Saskia Brandt
  • Sara Fachinger
  • Takayuki Tohge
  • Alisdair R. Fernie
  • Hans Peter Braun
  • Tatjana M. Hildebrandt

Research Organisations

External Research Organisations

  • Max Planck Institute of Molecular Plant Physiology (MPI-MP)

Details

Original languageEnglish
Article numbere0202153
JournalPLOS ONE
Volume13
Issue number8
Publication statusPublished - 9 Aug 2018

Abstract

Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level.

Keywords

    Aminohydrolases/metabolism, Arabidopsis/metabolism, Arabidopsis Proteins/metabolism, Carboxylic Acids/metabolism, Darkness, Glucosinolates/metabolism, Glycoside Hydrolases/metabolism, Light, Nitriles/metabolism, Plant Leaves/metabolism, Protein Processing, Post-Translational

ASJC Scopus subject areas

Cite this

Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. / Brandt, Saskia; Fachinger, Sara; Tohge, Takayuki et al.
In: PLOS ONE, Vol. 13, No. 8, e0202153, 09.08.2018.

Research output: Contribution to journalArticleResearchpeer review

Brandt S, Fachinger S, Tohge T, Fernie AR, Braun HP, Hildebrandt TM. Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLOS ONE. 2018 Aug 9;13(8):e0202153. doi: 10.1371/journal.pone.0202153, 10.15488/4163
Brandt, Saskia ; Fachinger, Sara ; Tohge, Takayuki et al. / Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. In: PLOS ONE. 2018 ; Vol. 13, No. 8.
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AU - Brandt, Saskia

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AU - Tohge, Takayuki

AU - Fernie, Alisdair R.

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AU - Hildebrandt, Tatjana M.

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