Details
Original language | English |
---|---|
Article number | e0202153 |
Journal | PLOS ONE |
Volume | 13 |
Issue number | 8 |
Publication status | Published - 9 Aug 2018 |
Abstract
Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level.
Keywords
- Aminohydrolases/metabolism, Arabidopsis/metabolism, Arabidopsis Proteins/metabolism, Carboxylic Acids/metabolism, Darkness, Glucosinolates/metabolism, Glycoside Hydrolases/metabolism, Light, Nitriles/metabolism, Plant Leaves/metabolism, Protein Processing, Post-Translational
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
- General
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: PLOS ONE, Vol. 13, No. 8, e0202153, 09.08.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves
AU - Brandt, Saskia
AU - Fachinger, Sara
AU - Tohge, Takayuki
AU - Fernie, Alisdair R.
AU - Braun, Hans Peter
AU - Hildebrandt, Tatjana M.
PY - 2018/8/9
Y1 - 2018/8/9
N2 - Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level.
AB - Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production. We investigated, whether the internal breakdown of glucosinolates, a major class of sulfur-containing secondary metabolites, might be an additional component of the carbohydrate starvation response in Arabidopsis thaliana (A. thaliana). The glucosinolate content of A. thaliana leaves was strongly reduced after seven days of darkness. We also detected a significant increase in the activity of myrosinase, the enzyme catalyzing the initial step in glucosinolate breakdown, coinciding with a strong induction of the main leaf myrosinase isoforms TGG1 and TGG2. In addition, nitrilase activity was increased suggesting a turnover via nitriles and carboxylic acids. Internal degradation of glucosinolates might also be involved in diurnal or developmental adaptations of the glucosinolate profile. We observed a diurnal rhythm for myrosinase activity in two-week-old plants. Furthermore, leaf myrosinase activity and protein abundance of TGG2 varied during plant development, whereas leaf protein abundance of TGG1 remained stable indicating regulation at the transcriptional as well as post-translational level.
KW - Aminohydrolases/metabolism
KW - Arabidopsis/metabolism
KW - Arabidopsis Proteins/metabolism
KW - Carboxylic Acids/metabolism
KW - Darkness
KW - Glucosinolates/metabolism
KW - Glycoside Hydrolases/metabolism
KW - Light
KW - Nitriles/metabolism
KW - Plant Leaves/metabolism
KW - Protein Processing, Post-Translational
UR - http://www.scopus.com/inward/record.url?scp=85052321785&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0202153
DO - 10.1371/journal.pone.0202153
M3 - Article
C2 - 30092103
AN - SCOPUS:85052321785
VL - 13
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 8
M1 - e0202153
ER -