Details
| Original language | English |
|---|---|
| Pages (from-to) | 491-508 |
| Number of pages | 18 |
| Journal | Photosynthesis research |
| Volume | 86 |
| Issue number | 3 |
| Publication status | Published - 1 Dec 2005 |
Abstract
The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.
Keywords
- Glucosinolates, Pathogen resistance, Sulfur metabolism, Sulfur-rich peptides, Thionin
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Agricultural and Biological Sciences(all)
- Plant Science
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Photosynthesis research, Vol. 86, No. 3, 01.12.2005, p. 491-508.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana
AU - Jost, Ricarda
AU - Altschmied, Lothar
AU - Bloem, Elke
AU - Bogs, Jochen
AU - Gershenzon, Jonathan
AU - Hähnel, Urs
AU - Hänsch, Robert
AU - Hartmann, Tanja
AU - Kopriva, Stanislav
AU - Kruse, Cordula
AU - Mendel, Ralf R.
AU - Papenbrock, Jutta
AU - Reichelt, Michael
AU - Rennenberg, Heinz
AU - Schnug, Ewald
AU - Schmidt, Ahlert
AU - Textor, Susanne
AU - Tokuhisa, Jim
AU - Wachter, Andreas
AU - Wirtz, Markus
AU - Rausch, Thomas
AU - Hell, Rüdiger
N1 - Funding information: The authors of the German Sulfur Research Group wish to thank the Deutsche Forschungs-gemeinschaft for funding of the Research Group 383. We are indebted to M. Bucher, ETH Zürich, I. Feussner, Universität Göttingen and F. Mauch, University of Fribourg, who donated cDNA clones for the array analysis, and to H. Bohlmann and K. Apel, ETH Zürich, for generous provision of the THI2.1 reporter line. We thank Christa Kallas for excellent technical assistance, M. Hajirezaei, Molecular Plant Physiology Group, IPK Gatersleben, for support with anion analysis and U. Scholz, Bioinformatics Group, IPK Gat-ersleben, for the web page development.
PY - 2005/12/1
Y1 - 2005/12/1
N2 - The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.
AB - The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.
KW - Glucosinolates
KW - Pathogen resistance
KW - Sulfur metabolism
KW - Sulfur-rich peptides
KW - Thionin
UR - http://www.scopus.com/inward/record.url?scp=29944433137&partnerID=8YFLogxK
U2 - 10.1007/s11120-005-7386-8
DO - 10.1007/s11120-005-7386-8
M3 - Article
C2 - 16307302
AN - SCOPUS:29944433137
VL - 86
SP - 491
EP - 508
JO - Photosynthesis research
JF - Photosynthesis research
SN - 0166-8595
IS - 3
ER -