Details
| Original language | English |
|---|---|
| Article number | 100008 |
| Journal | Engineering Microbiology |
| Volume | 2 |
| Issue number | 1 |
| Early online date | 26 Nov 2021 |
| Publication status | Published - Mar 2022 |
| Externally published | Yes |
Abstract
Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
Keywords
- Biocatalysis, Cyanobacteria, Hydroxyisocaproate dehydrogenase, Ketoacid dehydrogenase, Photobiotransformation, Synechocystis sp. PCC 6803
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Immunology and Microbiology(all)
- Microbiology
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In: Engineering Microbiology, Vol. 2, No. 1, 100008, 03.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH
AU - Jurkaš, Valentina
AU - Winkler, Christoph K.
AU - Poschenrieder, Silvan
AU - Oliveira, Paulo
AU - Pacheco, Catarina C.
AU - Ferreira, Eunice A.
AU - Weissensteiner, Florian
AU - Santis, Piera De
AU - Kara, Selin
AU - Kourist, Robert
AU - Tamagnini, Paula
AU - Kroutil, Wolfgang
N1 - Publisher Copyright: © 2021
PY - 2022/3
Y1 - 2022/3
N2 - Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
AB - Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy. Consequently, cyanobacteria, which can use light e.g., for generating NADPH, are in the focus of research. Previously, it has been shown that various heterologous redox enzymes could be expressed in these microorganisms. Here we demonstrated the successful inducer-free expression of α-keto-acid dehydrogenases (L-HicDH and D-HicDH) from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp. PCC 6803 ΔhoxYH mutant using replicative plasmids. While the L-HicDH showed poor activity limited by the amount of expressed enzyme, the D-HicDH was applied both in vivo and in vitro, transforming the selected α-keto acids to the corresponding optically pure (R)-α-hydroxy acids (ee >99%) in up to 53% and 90% conversion, respectively.
KW - Biocatalysis
KW - Cyanobacteria
KW - Hydroxyisocaproate dehydrogenase
KW - Ketoacid dehydrogenase
KW - Photobiotransformation
KW - Synechocystis sp. PCC 6803
UR - http://www.scopus.com/inward/record.url?scp=85137770966&partnerID=8YFLogxK
U2 - 10.1016/j.engmic.2021.100008
DO - 10.1016/j.engmic.2021.100008
M3 - Article
VL - 2
JO - Engineering Microbiology
JF - Engineering Microbiology
IS - 1
M1 - 100008
ER -