Details
| Original language | English |
|---|---|
| Pages (from-to) | 622-630 |
| Number of pages | 9 |
| Journal | Molecular Plant-Microbe Interactions |
| Volume | 21 |
| Issue number | 5 |
| Publication status | Published - 1 May 2008 |
| Externally published | Yes |
Abstract
Nitrogen fixation (NF) in legume nodules is very sensitive to environmental constraints. Nodule sucrose synthase (SS; EC 2.4.1.13) has been suggested to play a crucial role in those circumstances because its downregulation leads to an impaired glycolytic carbon flux and, therefore, a depletion of carbon substrates for bacteroids. In the present study, the likelihood of SS being regulated by oxidative signaling has been addressed by the in vivo supply of paraquat (PQ) to nodulated pea plants and the in vitro effects of oxidizing and reducing agents on nodule SS. PQ produced cellular redox imbalance leading to an inhibition of NF. This was preceded by the downregulation of SS gene expression, protein content, and activity. In vitro, oxidizing agents were able to inhibit SS activity and this inhibition was completely reversed by the addition of dithiothreitol. The overall results are consistent with a regulation model of nodule SS exerted by the cellular redox state at both the transcriptional and post-translational levels. The importance of such mechanisms for the regulation of NF in response to environmental stresses are discussed.
Keywords
- Carbon metabolism, Pisum sativum L.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
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In: Molecular Plant-Microbe Interactions, Vol. 21, No. 5, 01.05.2008, p. 622-630.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Evidence for transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state
AU - Marino, Daniel
AU - Hohnjec, Natalija
AU - Küster, Helge
AU - Moran, Jose F.
AU - González, Esther M.
AU - Arrese-Igor, Cesar
PY - 2008/5/1
Y1 - 2008/5/1
N2 - Nitrogen fixation (NF) in legume nodules is very sensitive to environmental constraints. Nodule sucrose synthase (SS; EC 2.4.1.13) has been suggested to play a crucial role in those circumstances because its downregulation leads to an impaired glycolytic carbon flux and, therefore, a depletion of carbon substrates for bacteroids. In the present study, the likelihood of SS being regulated by oxidative signaling has been addressed by the in vivo supply of paraquat (PQ) to nodulated pea plants and the in vitro effects of oxidizing and reducing agents on nodule SS. PQ produced cellular redox imbalance leading to an inhibition of NF. This was preceded by the downregulation of SS gene expression, protein content, and activity. In vitro, oxidizing agents were able to inhibit SS activity and this inhibition was completely reversed by the addition of dithiothreitol. The overall results are consistent with a regulation model of nodule SS exerted by the cellular redox state at both the transcriptional and post-translational levels. The importance of such mechanisms for the regulation of NF in response to environmental stresses are discussed.
AB - Nitrogen fixation (NF) in legume nodules is very sensitive to environmental constraints. Nodule sucrose synthase (SS; EC 2.4.1.13) has been suggested to play a crucial role in those circumstances because its downregulation leads to an impaired glycolytic carbon flux and, therefore, a depletion of carbon substrates for bacteroids. In the present study, the likelihood of SS being regulated by oxidative signaling has been addressed by the in vivo supply of paraquat (PQ) to nodulated pea plants and the in vitro effects of oxidizing and reducing agents on nodule SS. PQ produced cellular redox imbalance leading to an inhibition of NF. This was preceded by the downregulation of SS gene expression, protein content, and activity. In vitro, oxidizing agents were able to inhibit SS activity and this inhibition was completely reversed by the addition of dithiothreitol. The overall results are consistent with a regulation model of nodule SS exerted by the cellular redox state at both the transcriptional and post-translational levels. The importance of such mechanisms for the regulation of NF in response to environmental stresses are discussed.
KW - Carbon metabolism
KW - Pisum sativum L.
UR - http://www.scopus.com/inward/record.url?scp=43549117506&partnerID=8YFLogxK
U2 - 10.1094/MPMI-21-5-0622
DO - 10.1094/MPMI-21-5-0622
M3 - Article
C2 - 18393622
AN - SCOPUS:43549117506
VL - 21
SP - 622
EP - 630
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
SN - 0894-0282
IS - 5
ER -