Details
| Original language | English |
|---|---|
| Pages (from-to) | 981-990 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 101A |
| Issue number | 4 |
| Publication status | Published - 10 Sept 2012 |
| Externally published | Yes |
Abstract
One goal in biomaterials research is to limit the formation of connective tissue around the implant. Antiwetting surfaces are known to reduce ability of cells to adhere. Such surfaces can be achieved by special surface structures (lotus effect). Aim of the study was to investigate the feasibility for creating antiwetting surface structures on titanium and to characterize their effect on initial cell adhesion and proliferation. Titanium microstructures were generated using femtosecond-(fs-) laser pulses. Murine fibroblasts served as a model for connective tissue cells. Quantitative investigation of initial cell adhesion was performed using atomic force microscopy. Fluorescence microscopy was used for the characterization of cell-adhesion pattern, cell morphology, and proliferation. Water contact angle (WCA) measurements evinced antiwetting properties of laser-structured surfaces. However, the WCA was decreased in serum-containing medium. Initial cell adhesion to microstructured titanium was significantly promoted when compared with polished titanium. Microstructures did not influence cell proliferation on titanium surfaces. However, on titanium microstructures, cells showed a flattened morphology, and the cell orientation was biased according to the surface topography. In conclusion, antiwetting properties of surfaces were absent in the presence of serum and did not hinder adhesion and proliferation of NIH 3T3 fibroblasts.
Keywords
- Atomic force microscopy, Cell-substrate adhesion, Connective tissue growth, Femtosecond-laser, Titanium microstructure
ASJC Scopus subject areas
- Materials Science(all)
- Ceramics and Composites
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Materials Science(all)
- Metals and Alloys
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In: Journal of Biomedical Materials Research - Part A, Vol. 101A, No. 4, 10.09.2012, p. 981-990.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Evaluation of single-cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond-laser microstructured titanium surfaces
AU - Aliuos, Pooyan
AU - Fadeeva, Elena
AU - Badar, Muhammad
AU - Winkel, Andreas
AU - Mueller, Peter P.
AU - Warnecke, Athanasia
AU - Chichkov, Boris
AU - Lenarz, Thomas
AU - Reich, Uta
AU - Reuter, Guenter
PY - 2012/9/10
Y1 - 2012/9/10
N2 - One goal in biomaterials research is to limit the formation of connective tissue around the implant. Antiwetting surfaces are known to reduce ability of cells to adhere. Such surfaces can be achieved by special surface structures (lotus effect). Aim of the study was to investigate the feasibility for creating antiwetting surface structures on titanium and to characterize their effect on initial cell adhesion and proliferation. Titanium microstructures were generated using femtosecond-(fs-) laser pulses. Murine fibroblasts served as a model for connective tissue cells. Quantitative investigation of initial cell adhesion was performed using atomic force microscopy. Fluorescence microscopy was used for the characterization of cell-adhesion pattern, cell morphology, and proliferation. Water contact angle (WCA) measurements evinced antiwetting properties of laser-structured surfaces. However, the WCA was decreased in serum-containing medium. Initial cell adhesion to microstructured titanium was significantly promoted when compared with polished titanium. Microstructures did not influence cell proliferation on titanium surfaces. However, on titanium microstructures, cells showed a flattened morphology, and the cell orientation was biased according to the surface topography. In conclusion, antiwetting properties of surfaces were absent in the presence of serum and did not hinder adhesion and proliferation of NIH 3T3 fibroblasts.
AB - One goal in biomaterials research is to limit the formation of connective tissue around the implant. Antiwetting surfaces are known to reduce ability of cells to adhere. Such surfaces can be achieved by special surface structures (lotus effect). Aim of the study was to investigate the feasibility for creating antiwetting surface structures on titanium and to characterize their effect on initial cell adhesion and proliferation. Titanium microstructures were generated using femtosecond-(fs-) laser pulses. Murine fibroblasts served as a model for connective tissue cells. Quantitative investigation of initial cell adhesion was performed using atomic force microscopy. Fluorescence microscopy was used for the characterization of cell-adhesion pattern, cell morphology, and proliferation. Water contact angle (WCA) measurements evinced antiwetting properties of laser-structured surfaces. However, the WCA was decreased in serum-containing medium. Initial cell adhesion to microstructured titanium was significantly promoted when compared with polished titanium. Microstructures did not influence cell proliferation on titanium surfaces. However, on titanium microstructures, cells showed a flattened morphology, and the cell orientation was biased according to the surface topography. In conclusion, antiwetting properties of surfaces were absent in the presence of serum and did not hinder adhesion and proliferation of NIH 3T3 fibroblasts.
KW - Atomic force microscopy
KW - Cell-substrate adhesion
KW - Connective tissue growth
KW - Femtosecond-laser
KW - Titanium microstructure
UR - http://www.scopus.com/inward/record.url?scp=84876196909&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.34401
DO - 10.1002/jbm.a.34401
M3 - Article
C2 - 22965938
AN - SCOPUS:84876196909
VL - 101A
SP - 981
EP - 990
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 4
ER -